The transcriptional response of the Pacific oyster Crassostrea gigas under simultaneous bacterial and heat stresses

doi:10.1016/j.dci.2019.01.006

IOCAS-IR > 实验海洋生物学重点实验室

	The transcriptional response of the Pacific oyster Crassostrea gigas under simultaneous bacterial and heat stresses
	Zhang, Huan 1,4; Wang, Hao 1,4; Chen, Hao1,4 ; Wang, Mengqiang1,2 ; Zhou, Zhi 2; Qiu, Limei 1,2; Wang, Lingling 3; Song, Linsheng 3
	2019-05-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	94 页码:1-10
通讯作者	Wang, Hao() ; Song, Linsheng([email protected])
摘要	Bacterial infection and heat stress are considered as two major environmental threats for the aquaculture industry of oyster Crassostrea gigas. In the present study, the expression profiles of mRNA transcripts in the hemocytes of oysters under bacterial challenge and heat stress were examined by next-generation sequencing. There were 21,095, 21,957 and 21,141 transcripts identified in the hemocytes of oysters from three groups, respectively, including control group (designated as Con group), Vibrio splendidus challenge group (Bac group), and bacterial and heat stress combined treatment group (BacHeat group). There were 4610, 5093 and 5149 differentially expressed transcripts (DTs) in the three pairwise comparisons Con/Bac, Con/BacHeat and Bac/BacHeat, respectively. The main enriched GO terms in biological process category of the DTs included the metabolic processes, cellular process, response to stimulus and immune system process. The expression patterns of DTs involved in pattern recognition, immune signal transduction and energy metabolic indicated that the immune response to bacterial challenge was disturbed under acute heat stress, which was also confirmed by quantitative real-time PCR. The neuroendocrine immunomodulation, especially the catecholaminergic regulation, played indispensable roles in stress response. The total energy reserves as well as cellular energy allocation (CEA) in hepatopancreas of oysters decreased remarkably especially in BacHeat group, while the energy consumption generally increased, suggesting that the immune defense against the simultaneous stimulation of pathogen and heat stress imposed greater costs on oyster's energy expenditure than a single stressor. These results above indicated that, the heat stress disturbed the normal expression of genes involved in immune response and energy metabolism, accelerated energy consumption and broke the metabolic balance, leading to a decline in resilience to infection and mass mortality of oyster in summer.
关键词	Crassostrea gigas Transcriptome analysis Vibrio splendidus Immune challenge Heat stress Energy metabolic
DOI	10.1016/j.dci.2019.01.006
收录类别	SCI
语种	英语
资助项目	[2018YFD0900606]###237; National Science Foundation of China[41476145,31530069]###236; National Science Foundation of China[41476145,31530069]; [2018YFD0900606]
WOS研究方向	Fisheries ; Immunology ; Zoology
WOS类目	Fisheries ; Immunology ; Zoology
WOS记录号	WOS:000460826100001
出版者	ELSEVIER SCI LTD
引用统计	被引频次：29[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/155244
专题	实验海洋生物学重点实验室深海极端环境与生命过程研究中心
通讯作者	Wang, Hao; Song, Linsheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 3.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, 52 Heishijiao St Dalian, Dalian 116023, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
第一作者单位	深海极端环境与生命过程研究中心; 中国科学院海洋大科学研究中心
通讯作者单位	深海极端环境与生命过程研究中心; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Zhang, Huan,Wang, Hao,Chen, Hao,et al. The transcriptional response of the Pacific oyster Crassostrea gigas under simultaneous bacterial and heat stresses[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2019,94:1-10.
APA	Zhang, Huan.,Wang, Hao.,Chen, Hao.,Wang, Mengqiang.,Zhou, Zhi.,...&Song, Linsheng.(2019).The transcriptional response of the Pacific oyster Crassostrea gigas under simultaneous bacterial and heat stresses.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,94,1-10.
MLA	Zhang, Huan,et al."The transcriptional response of the Pacific oyster Crassostrea gigas under simultaneous bacterial and heat stresses".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 94(2019):1-10.

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