Muscle fibre type composition in the lateral muscle of olive flounder Paralichthys olivaceus

doi:10.1016/j.acthis.2018.10.002

IOCAS-IR > 实验海洋生物学重点实验室

	Muscle fibre type composition in the lateral muscle of olive flounder Paralichthys olivaceus
	Jiao, Shuang 1,2,3; Tan, Xungang1,2,3 ; Sui, Yulei1,2,3,4 ; You, Feng 1,2,3
	2019
发表期刊	ACTA HISTOCHEMICA
ISSN	0065-1281
卷号	121 期号:1 页码:1-6
通讯作者	Tan, Xungang([email protected])
摘要	In this paper, a combined-method study has been made on the lateral muscle of the teleost olive flounder Paralichthys olivaceus in just-hatched and adult stages. In just-hatched stage, both slow and fast muscle fibres were detected: (1) in situ hybridization analysis indicated that slow and fast myosin heavy chain genes were specifically expressed in the superficial and deep part of the myotomal muscle, respectively; (2) immunohistochemistry analysis showed that fibres in the deep part reacted with anti-fast myosin antibody F310; (3) western blot analysis detected a weak expression of slow myosin and a strong expression of fast myosin. In adult stage, the slow and fast muscle fibres had their own distribution characteristics: (1) hematoxylin/eosin staining showed the histological characteristics of the muscle fibre composition; (2) histochemical observations showed that the deep muscle fibres, and some fibres near the epidermis, contain alkali-stable myofibrillar ATPase activity; (3) immunohistochemistry analysis indicated that all the deep muscle fibres reacted with F310 antibody and some fibres in the superficial layer of muscle also reacted with F310; (4) western blot analysis showed that fast myosin was expressed both in the blended muscles (the mix of superficial and deep muscles) and deep muscles, while slow myosin was mainly expressed in the blended muscles. These findings suggested that both slow and fast muscle fibres existed in the musculature of the olive flounder in just-hatched and adult stages. Notably, the adult fast fibres also exist in the superficial layer of the muscle.
关键词	Olive flounder Muscle fibre Fast muscle Slow muscle
DOI	10.1016/j.acthis.2018.10.002
收录类别	SCI
语种	英语
资助项目	National Infrastructure of Fishery Germplasm Resource[2017DKA30470]###225; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02]###218; National Flatfish Industry System Construction Programme of China[nycytx-50]###224; National Natural Science Foundation of China[31672636]###223; National Natural Science Foundation of China[31502146]###222; National Natural Science Foundation of China[31502146]; National Natural Science Foundation of China[31672636]; National Flatfish Industry System Construction Programme of China[nycytx-50]; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02]; National Infrastructure of Fishery Germplasm Resource[2017DKA30470]
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000461000600001
出版者	ELSEVIER GMBH
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/155235
专题	实验海洋生物学重点实验室
通讯作者	Tan, Xungang
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Jiao, Shuang,Tan, Xungang,Sui, Yulei,et al. Muscle fibre type composition in the lateral muscle of olive flounder Paralichthys olivaceus[J]. ACTA HISTOCHEMICA,2019,121(1):1-6.
APA	Jiao, Shuang,Tan, Xungang,Sui, Yulei,&You, Feng.(2019).Muscle fibre type composition in the lateral muscle of olive flounder Paralichthys olivaceus.ACTA HISTOCHEMICA,121(1),1-6.
MLA	Jiao, Shuang,et al."Muscle fibre type composition in the lateral muscle of olive flounder Paralichthys olivaceus".ACTA HISTOCHEMICA 121.1(2019):1-6.

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