大型海藻微球体的反应器培养和应用基础研究

IOCAS-IR > 海洋环流与波动重点实验室

	大型海藻微球体的反应器培养和应用基础研究
其他题名	Cultures of the microsphere of macroalgae in photobioreactor and its basic application studies
	王金霞
学位类型	博士
	2009-01-10
学位授予单位	中国科学院海洋研究所
学位授予地点	海洋研究所
关键词	大型海藻微球体光生物反应器光能传输
摘要	构建海藻微球体以获得适宜的藻体形态和研究了解微球体系统的光能传输情况以提高光能利用效率，这是实现海藻的生物反应器高密度培养的二个关键问题。本文以蜈蚣藻、多管藻、孔石莼3种海藻的丝状体无性系为起始材料，分别构建了绒球状、网状和簇生这3类微球体，通过研究其形态建成的原理和方法，提出构建各类海藻的微球体的普适性原理和技术。采用自制的光合测定装置、光温双梯度培养装置和藻类生物反应器等研究了微球体的光合特性、培养条件、营养盐吸收，建立了相关的研究方法。以比较典型的孔石莼簇生状微球体为代表，进行了微球体光能利用情况的研究，着重分析研究了长期以来一直被忽视的微球体内部的光衰减情况，建立了光衰减模型；将反应器表面至藻体表面和藻体内部的光能利用情况相结合，全面了解反应器培养中的光能传输问题。研究结果为改进光生物反应器和培养技术，提高光能利用效率，实现高密度培养提供了依据。同时，这些海藻在细胞工程育苗、生态工程和天然产物生产等方面有着重要的应用价值，分别对其育苗、无机氮磷吸收、藻胆蛋白含量等方面进行了初步研究，为大型海藻的生物反应器培养应用于海藻良种化育苗养殖、药物等高值化产品生产和水产养殖水处理等开辟了新途径。
其他摘要	There are two problem to realize the high-density culture of macroalgae in photo-bioreactor. One is to develop algal microsphere systems suitable for bioreactor cultivation; the other is to know the light delivery in algal microshphe suspension cultures. Three types of algal microsphere were developed from the clonings of Grateloupia filicina, Polysiphonia urceolata and Ulva pertusa, which are flossy, netty and fascicular respectively. The morphology developments were studies and the universality principles and methods to develop microsphere were proposed. A apparatus for detect oxygen released by photosynthesis, a crossed-gradient of temperature and light intensity cultural apparatus, a photo-bioreactor were assembled to study the photosynthetic character, culture condition, nutrition absord of algal microsphere. The corrective research methods were found. The light utility of algal microsphere was studied with fascicular microsphere of U. pertusa for its typical form. The research put emphasis on the attenuation of light in the single microsphere, which had always been neglected. A model of light attenuation in algal microshpere was erected. Only the light delivery from illuminated surface to microsphere surface combined with the inner microsphere could explain the light delivery and utility by algae in photo bioreactor. The results provided the basis of photo-bioreactor vessel design, the mode of algal cultures, high efficient utility of light and high-density culture of macroalgae. Meanwhile, the algae are valuable to aquaculture, aquatic environment remedy and natural bioactive product. The utilities of the algae on seaweed breeding, absorbing nitrogen and phosphate and producing phycobiliproteins were studied primarily, which can be used in algal cultivation, integrate-aquaculture and bioactive products widely.
页数	121
语种	中文
文献类型	学位论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/1483
专题	海洋环流与波动重点实验室
推荐引用方式 GB/T 7714	王金霞. 大型海藻微球体的反应器培养和应用基础研究[D]. 海洋研究所. 中国科学院海洋研究所,2009.

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