Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Cytochemical identification of turbot myeloperoxidase-positive granulocytes by potassium iodide and oxidized pyronine Y staining | |
Chi, Heng1,4; Wen, Li-lian1,3; Sui, Zhi-Hai1,3; Sun, Qing-Lei1,2 ; Sun, Li1,2,4
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| 2017-12-01 | |
| 发表期刊 | TISSUE & CELL
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| 卷号 | 49期号:6页码:751-755 |
| 文章类型 | Article |
| 摘要 | Cytomorphological and cytochemical staining are important methods for the identification of cell types, in particular in fish which often lack biological tools such as specific antibodies. Myeloperoxidase (MPO) is usually used as an intracellular marker of neutrophil accumulation in tissues and a marker of neutrophil activity in plasma. In this study, we reported a potassium iodide and oxidized pyronine Y (KI-PyY) staining method for rapid and highly sensitive detection of MPO-positive cells in turbot blood, peritoneum, and tissues. MPO-positive cells, which mostly represented neutrophils, were stained brown and clearly distinguished from other cells, such as lymphocytes, monocytes, and macrophages, which were stained pink. Following bacterial stimulation, the proportions of neutrophils were 27.49% and 38.05% in peripheral blood leukocytes and peritoneum, respectively, judging by the stained MPO. Kidney granulocytes contained abundant MPO-positive cells which were probably immature neutrophils with low expression of MPO. It is noteworthy that MPO-positive cells were detected in the tissue sections of kidney, spleen, and gut, with distribution profiles specific to each tissue. However, the cell morphology was not distinct in the stained tissue sections. These results indicate that the KI-PyY staining method is highly sensitive, applicable to different types of samples, and will be useful for the study of neutrophils in different compartments of fish. |
| 关键词 | Cytochemical Identification Ki-pyy Turbot Mpo-positive Granulocytes |
| DOI | 10.1016/j.tice.2017.10.008 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000417042700015 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/143292 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室; 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Chi, Heng,Wen, Li-lian,Sui, Zhi-Hai,et al. Cytochemical identification of turbot myeloperoxidase-positive granulocytes by potassium iodide and oxidized pyronine Y staining[J]. TISSUE & CELL,2017,49(6):751-755. |
| APA | Chi, Heng,Wen, Li-lian,Sui, Zhi-Hai,Sun, Qing-Lei,&Sun, Li.(2017).Cytochemical identification of turbot myeloperoxidase-positive granulocytes by potassium iodide and oxidized pyronine Y staining.TISSUE & CELL,49(6),751-755. |
| MLA | Chi, Heng,et al."Cytochemical identification of turbot myeloperoxidase-positive granulocytes by potassium iodide and oxidized pyronine Y staining".TISSUE & CELL 49.6(2017):751-755. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Cytochemical identif（834KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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