| Degradation of Polysaccharides from Grateloupia filicina and Their Antiviral Activity to Avian Leucosis Virus Subgroup J | |
Sun, Yuhao1,2,3 ; Chen, Xiaolin1,3; Cheng, Ziqiang4; Liu, Song1,3; Yu, Huahua1,3; Wang, Xueqin1,3; Li, Pengcheng1,3
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| 2017-11-01 | |
| 发表期刊 | MARINE DRUGS
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| 卷号 | 15期号:11 |
| 文章类型 | Article |
| 摘要 | In this study, polysaccharides from Grateloupia filicinia (GFP) were extracted and several low molecular weight (Mw) G. filicina polysaccharides (LGFPs) were prepared by the hydrogen peroxide (H2O2) oxidation method. Additionally, the effect of different experimental conditions on the degradation of GFP was determined. Results showed that the GFP degradation rate was positively related to H2O2 concentration and temperature, and negatively related to pH. Chemical analysis and Fourier transform infrared spectra (FT-IR) of GFP and LGFPs showed that the degradation caused a slight decrease of total sugar and sulfate content. However, there was no obvious change for monosaccharide contents. Then, the anti-ALV-J activity of GFP and LGFPs were determined in vitro. Results revealed that all of the samples could significantly inhibit ALV-J and lower Mw LGFPs exhibited a stronger suppression, and that the fraction LGFP-3 with Mw 8.7 kDa had the best effect. In addition, the reaction phase assays showed that the inhibition effect was mainly because of the blocking virus adsorption to host cells. Moreover, real-time PCR, western-blot, and IFA were further applied to evaluate the blocking effects of LGFP-3. Results showed that the gene relative expression and gp85 protein for LGFPS-3 groups were all reduced. Data from IFA showed that there was less virus infected cells for 1000 and 200 g/mL LGFPS-3 groups when compared to virus control. Therefore, lower Mw polysaccharides from G. filicina might supply a good choice for ALV-J prevention and treatment. |
| 关键词 | Grateloupia Filicina Degradation Low Molecular Weight Polysaccharides Antiviral Activity Alv-j |
| DOI | 10.3390/md15110345 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000416600200016 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/143282 |
| 专题 | 海洋生物技术研发中心 实验海洋生物学重点实验室 海洋环境工程技术研究发展中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, 7 Nanhai Rd, Qingdao 266071, Peoples R China 4.Shandong Agr Univ, Coll Anim Sci & Vet Med, 61 Daizong Rd, Tai An 271018, Shandong, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Sun, Yuhao,Chen, Xiaolin,Cheng, Ziqiang,et al. Degradation of Polysaccharides from Grateloupia filicina and Their Antiviral Activity to Avian Leucosis Virus Subgroup J[J]. MARINE DRUGS,2017,15(11). |
| APA | Sun, Yuhao.,Chen, Xiaolin.,Cheng, Ziqiang.,Liu, Song.,Yu, Huahua.,...&Li, Pengcheng.(2017).Degradation of Polysaccharides from Grateloupia filicina and Their Antiviral Activity to Avian Leucosis Virus Subgroup J.MARINE DRUGS,15(11). |
| MLA | Sun, Yuhao,et al."Degradation of Polysaccharides from Grateloupia filicina and Their Antiviral Activity to Avian Leucosis Virus Subgroup J".MARINE DRUGS 15.11(2017). |
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| Degradation of Polys（5976KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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