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| Peritrophin-like protein from Litopenaeus vannamei (LvPT) involved in white spot syndrome virus (WSSV) infection in digestive tract challenged with reverse gavage | |
Xie Shijun1,2 ; Li Fuhua1,3; Zhang Xiaojun1; Zhang Jiquan1; Xiang Jianhai1,3
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| 2017-11-01 | |
| 发表期刊 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| 卷号 | 35期号:6页码:1524-1530 |
| 文章类型 | Article |
| 摘要 | The peritrophic membrane plays an important role in the defense system of the arthropod gut. The digestive tract is considered one of the major tissues targeted by white spot syndrome virus (WSSV) in shrimp. In this study, the nucleotide sequence encoding peritrophin-like protein of Litopenaeus vannamei (LvPT) was amplified from a yeast two-hybrid library of L. vannamei. The epitope peptide of LvPT was predicted with the GenScript OptimumAntigen (TM) design tool. An anti-LvPT polyclonal antibody was produced and shown to specifically bind a band at similar to 27 kDa, identified as LvPT. The LvPT protein was expressed and its concentration determined. LvPT dsRNA (4 mu g per shrimp) was used to inhibit LvPT expression in shrimp, and a WSSV challenge experiment was then performed with reverse gavage. The pleopods, stomachs, and guts were collected from the shrimp at 0, 24, 48, and 72 h post-infection (hpi). Viral load quantification showed that the levels of WSSV were significantly lower in the pleopods, stomachs, and guts of shrimp after LvPT dsRNA interference than in those of the controls at 48 and 72 hpi. Our results imply that LvPT plays an important role during WSSV infection of the digestive tract. |
| 关键词 | Litopenaeus Vannamei Digestive Tract Peritrophin-like Protein Dsrna Interference Binding Specificity White Spot Syndrome Virus (Wssv) Reverse Gavage |
| DOI | 10.1007/s00343-017-6109-2 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000415810900023 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/143280 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Xie Shijun,Li Fuhua,Zhang Xiaojun,et al. Peritrophin-like protein from Litopenaeus vannamei (LvPT) involved in white spot syndrome virus (WSSV) infection in digestive tract challenged with reverse gavage[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2017,35(6):1524-1530. |
| APA | Xie Shijun,Li Fuhua,Zhang Xiaojun,Zhang Jiquan,&Xiang Jianhai.(2017).Peritrophin-like protein from Litopenaeus vannamei (LvPT) involved in white spot syndrome virus (WSSV) infection in digestive tract challenged with reverse gavage.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,35(6),1524-1530. |
| MLA | Xie Shijun,et al."Peritrophin-like protein from Litopenaeus vannamei (LvPT) involved in white spot syndrome virus (WSSV) infection in digestive tract challenged with reverse gavage".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 35.6(2017):1524-1530. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| eritrophin-like prot（523KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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