| A novel fluorinated thiosemicarbazone derivative-2-(3,4-difluorobenzylidene) hydrazinecarbothioamide induces apoptosis in human A549 lung cancer cells via ROS-mediated mitochondria-dependent pathway | |
Zhao, Yue1,2,3; Guo, Chuanlong2,3,4; Wang, Lijun2,3; Wang, Shuaiyu2,3; Li, Xiangqian2,3; Jiang, Bo2,3; Wu, Ning2,3; Guo, Shuju2,3; Zhang, Renshuai2,3; Liu, Kun1 ; Shi, Dayong2,3,4
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| 2017-09-09 | |
| 发表期刊 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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| 卷号 | 491期号:1页码:65-71 |
| 文章类型 | Article |
| 摘要 | Thiosemicarbazone, a class of compounds with excellent biological activity, especially antitumor activity, have attracted wide attention. In this study, a novel fluorinated thiosemicarbazone derivative, 2-(3,4difluorobenzylidene) hydrazinecarbothioamide (compound 1) was synthesized and its antitumor activities were further investigated on a non-small cell lung cancer cell line (A549) along with its underlying mechanisms. Compound 1 showed significant anti-proliferative activity on A549 cells, which was further proved by colony formation experiment. Compound 1 also inhibits the invasion of A549 cells in a trans-well culture system. Moreover, compound 1 markedly induced apoptosis on A549 cells, and the ratio of Bcl-2/Bax was decreased while the amount of p53, Cleaved-Caspase 3 and Cleaved-PARP expression were increased significantly. Compound 1 decreased the mitochondrial membrane potential, while the content of reactive oxygen was increased obviously. It is revealed that compound 1 mediated cell cycle arrest in GO/G1 phase by reducing G1 phase dependent proteins, CDK4 and Cyclin Dl. As a result, it is indicated that compound 1 induced apoptosis on A549 cells was realized by regulating ROS-mediated mitochondria-dependent signaling pathway. (C) 2017 Elsevier Inc. All rights reserved. |
| 关键词 | Thiosemicarbazone Anticancer 2-(3 Apoptosis 4-difluorobenzylidene) Hydrazinecarbothioamide Ros Mitochondria-dependent Pathway |
| DOI | 10.1016/j.bbrc.2017.07.042 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000408518700011 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/143157 |
| 专题 | 海洋生物技术研发中心 |
| 作者单位 | 1.Qingdao Univ, Sch Pharm, Dept Pharmaceut Anal, Qingdao 266021, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhao, Yue,Guo, Chuanlong,Wang, Lijun,et al. A novel fluorinated thiosemicarbazone derivative-2-(3,4-difluorobenzylidene) hydrazinecarbothioamide induces apoptosis in human A549 lung cancer cells via ROS-mediated mitochondria-dependent pathway[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2017,491(1):65-71. |
| APA | Zhao, Yue.,Guo, Chuanlong.,Wang, Lijun.,Wang, Shuaiyu.,Li, Xiangqian.,...&Shi, Dayong.(2017).A novel fluorinated thiosemicarbazone derivative-2-(3,4-difluorobenzylidene) hydrazinecarbothioamide induces apoptosis in human A549 lung cancer cells via ROS-mediated mitochondria-dependent pathway.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,491(1),65-71. |
| MLA | Zhao, Yue,et al."A novel fluorinated thiosemicarbazone derivative-2-(3,4-difluorobenzylidene) hydrazinecarbothioamide induces apoptosis in human A549 lung cancer cells via ROS-mediated mitochondria-dependent pathway".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 491.1(2017):65-71. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| A novel fluorinated （1849KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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