Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| High temperature causes masculinization of genetically female olive flounder (Paralichthys olivaceus) accompanied by primordial germ cell proliferation detention | |
Wang, Xueying1,2 ; Liu, Qinghua1,2; Xiao, Yongshuang1,2; Yang, Yang1,2,3; Wang, Yanfeng1,2; Song, Zongcheng4; You, Feng1,2; An, Hao1,2,3; Li, Jun1,2
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| 2017-10-01 | |
| 发表期刊 | AQUACULTURE
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| 卷号 | 479页码:808-816 |
| 文章类型 | Article |
| 摘要 | Sex determination is not only resort to a single genetic cascade but also regulated along a continuum of environmental and heritable factors in many fish species. Temperature is one of the most important non-genetic sex differentiation elements. Previous studies regarding high temperature masculinization of flatfish typically focused on sex ratios and reproductive endocrinology, but little was known about germ cells during the process. In this study, we investigated the dynamic changes of germ cells in terms of morphology and number employing serial-sectioning and the molecular investigation of expression of dnd and amh genes by qRT-PCR for qualitative and quantitative analysis of germ cell proliferation during sexual differentiation. Moreover, the proliferation activity of primordial germ cells was investigated using PCNA (proliferating cell nuclear antigen) immunohistochemistry. |
| 关键词 | Paralichthys Olivaceus High Temperature Germ Cell Sex Differentiation |
| DOI | 10.1016/j.aquaculture.2017.07.019 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000408034700099 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/143135 |
| 专题 | 实验海洋生物学重点实验室 海洋生物技术研发中心 胶州湾海洋生态系统国家野外研究站 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai 264200, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Wang, Xueying,Liu, Qinghua,Xiao, Yongshuang,et al. High temperature causes masculinization of genetically female olive flounder (Paralichthys olivaceus) accompanied by primordial germ cell proliferation detention[J]. AQUACULTURE,2017,479:808-816. |
| APA | Wang, Xueying.,Liu, Qinghua.,Xiao, Yongshuang.,Yang, Yang.,Wang, Yanfeng.,...&Li, Jun.(2017).High temperature causes masculinization of genetically female olive flounder (Paralichthys olivaceus) accompanied by primordial germ cell proliferation detention.AQUACULTURE,479,808-816. |
| MLA | Wang, Xueying,et al."High temperature causes masculinization of genetically female olive flounder (Paralichthys olivaceus) accompanied by primordial germ cell proliferation detention".AQUACULTURE 479(2017):808-816. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| High temperature cau（2135KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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