Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Development and Application of a Quantitative Real-time Polymerase Chain Reaction Assay for the Detection of Aeromonas salmonicida | |
Du, Yishuai1; Liu, Ying1 ; Xiao, Peng1; Meng, Lingjie1,2; Liu, Pengfei1,2
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| 2017-08-01 | |
| 发表期刊 | JOURNAL OF THE WORLD AQUACULTURE SOCIETY
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| 卷号 | 48期号:4页码:574-582 |
| 文章类型 | Article |
| 摘要 | A rapid, economical, specific, and sensitive quantitative real-time polymerase chain reaction (qPCR) assay coupled with SYBR Green I chemistry was developed for the quantitative detection of Aeromonas salmonicida from farmed Atlantic salmon, Salmo salar, with the symptoms of furunculosis. The set of primers designed from the virulence array protein (vapA) gene was specific to A. salmonicida. Compared with the conventional PCR, qPCR had a lower detection limit of 5.6 copies of the positive plasmids. The standard curve, which showed the relationship between the copies of A. salmonicida and its quantification cycle (C-q) value, could be described as follows: log (copies of A. salmonicida)=-0.3213 C-q + 10.721. The quantitative detection of copies of A. salmonicida in different tissues of the moribund Atlantic salmon showed that A. salmonicida could be detected in all tissues; the spleen contained the largest number of A. salmonicida and then the kidney. These results suggest that the qPCR assay reported here is a specific, sensitive, and quantitative method for detecting A. salmonicida. It can be used for the routine tests of A. salmonicida in local aquaculture enterprise and for the research of infection routes of A. salmonicida to Atlantic salmon. |
| 关键词 | Atlantic Salmon Aeromonas Salmonicida Real-time Pcr Sybr Green i Quantitative Detection |
| DOI | 10.1111/jwas.12395 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000407075000005 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/143089 |
| 专题 | 实验海洋生物学重点实验室 海洋生物技术研发中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Earth & Environm Sci Coll, Beijing 10049, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Du, Yishuai,Liu, Ying,Xiao, Peng,et al. Development and Application of a Quantitative Real-time Polymerase Chain Reaction Assay for the Detection of Aeromonas salmonicida[J]. JOURNAL OF THE WORLD AQUACULTURE SOCIETY,2017,48(4):574-582. |
| APA | Du, Yishuai,Liu, Ying,Xiao, Peng,Meng, Lingjie,&Liu, Pengfei.(2017).Development and Application of a Quantitative Real-time Polymerase Chain Reaction Assay for the Detection of Aeromonas salmonicida.JOURNAL OF THE WORLD AQUACULTURE SOCIETY,48(4),574-582. |
| MLA | Du, Yishuai,et al."Development and Application of a Quantitative Real-time Polymerase Chain Reaction Assay for the Detection of Aeromonas salmonicida".JOURNAL OF THE WORLD AQUACULTURE SOCIETY 48.4(2017):574-582. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Development and Appl（385KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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