Genome Sequence of Pseudomonas stutzeri 273 and Identification of the Exopolysaccharide EPS273 Biosynthesis Locus

doi:10.3390/md15070218

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	Genome Sequence of Pseudomonas stutzeri 273 and Identification of the Exopolysaccharide EPS273 Biosynthesis Locus
	Wu, Shimei 1; Zheng, Rikuan2,3,4 ; Sha, Zhenxia 1; Sun, Chaomin 2,4
	2017-07-01
发表期刊	MARINE DRUGS
卷号	15 期号:7
文章类型	Article
摘要	Pseudomonas stutzeri 273 is a marine bacterium producing exopolysaccharide 273 (EPS273) with high anti-biofilm activity against P. aeruginosa PAO1. Here, the complete genome of P. stutzeri 273 was sequenced and the genome contained a circular 5.03 Mb chromosome. With extensive analysis of the genome, a genetic locus containing 18 genes was predicted to be involved in the biosynthesis of EPS273. In order to confirm this prediction, two adjacent genes (eps273-H and eps273-I) encoding glycosyltransferases and one gene (eps273-O) encoding tyrosine protein kinase within the genetic locus were deleted and biosynthesis of EPS273 was checked in parallel. The molecular weight profile of EPS purified from the mutant D eps273-HI was obviously different from that purified from wild-type P. stutzeri 273, while the corresponding EPS was hardly detected from the mutant D eps273-O, which indicated the involvement of the proposed 18-gene cluster in the biosynthesis of EPS273. Moreover, the mutant D eps273-HI had the biofilm formed earlier compared with the wild type, and the mutant D eps273-O almost completely lost the ability of biofilm formation. Therefore, EPS273 might facilitate the biofilm formation for its producing strain P. stutzeri 273 while inhibiting the biofilm formation of P. aeruginosa PAO1. This study can contribute to better understanding of the biosynthesis of EPS273 and disclose the biological function of EPS273 for its producing strain P. stutzeri 273.
关键词	Genome Pseudomonas Stutzeri Exopolysaccharide Biosynthesis Biofilm
DOI	10.3390/md15070218
收录类别	SCI
语种	英语
WOS记录号	WOS:000406685900026
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/143086
专题	实验海洋生物学重点实验室
作者单位	1.Qingdao Univ, Coll Life Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Wu, Shimei,Zheng, Rikuan,Sha, Zhenxia,et al. Genome Sequence of Pseudomonas stutzeri 273 and Identification of the Exopolysaccharide EPS273 Biosynthesis Locus[J]. MARINE DRUGS,2017,15(7).
APA	Wu, Shimei,Zheng, Rikuan,Sha, Zhenxia,&Sun, Chaomin.(2017).Genome Sequence of Pseudomonas stutzeri 273 and Identification of the Exopolysaccharide EPS273 Biosynthesis Locus.MARINE DRUGS,15(7).
MLA	Wu, Shimei,et al."Genome Sequence of Pseudomonas stutzeri 273 and Identification of the Exopolysaccharide EPS273 Biosynthesis Locus".MARINE DRUGS 15.7(2017).

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