Institutional Repository of Key Laboratory of Marine Ecology & Environmental Sciences, CAS
| Carbohydrate and amino acids metabolic response to heat stress in the intestine of the sea cucumber Apostichopus japonicus | |
Xu, Dongxue1 ; Zhou, Shun1; Yang, Hongsheng2
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| 2017-12-01 | |
| 发表期刊 | AQUACULTURE RESEARCH
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| 卷号 | 48期号:12页码:5883-5891 |
| 文章类型 | Article |
| 摘要 | The sea cucumber Apostichopus japonicus is an economic species mainly distributed along the coast of northern China, south-eastern Russia, Japan, Republic of Korea and Democratic People's Republic of Korea. Aquaculture industry of A.japonicus has been facing severe challenge of high temperature. In this study, we studied the mRNA expression profiles of eight key metabolic enzymes involved in carbohydrate and amino acids metabolism in A.japonicus under heat stress. The expression of hexokinase, pyruvate kinase and malate dehydrogenase showed downregulated response to heat stress, while the expression of glutamate dehydrogenase, alanine transaminase and branched-chain aminotransferase showed upregulated response. In addition, the expression of lactate dehydrogenase and glutamate synthase showed no significant difference. We also applied H-1 NMR-based metabolomics to investigate metabolic changes in the intestine tissue of A. japonicus under heat stress, the results of which revealed nine increased and 10 decreased metabolites in the heat stress group. These response genes and metabolites have potential to become markers for identifying severity of heat stress. More importantly, our findings suggest significant links between gene expression and metabolites changing, highlighting regulation networks of carbohydrate and amino acid metabolism in heat-stressed A. japonicus. |
| 关键词 | Apostichopus Japonicus Carbohydrate And Amino Acid Mechanism Heat Stress Metabolic Enzymes Metabolomics |
| DOI | 10.1111/are.13411 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000414941000019 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/143038 |
| 专题 | 海洋生态与环境科学重点实验室 |
| 作者单位 | 1.Qingdao Agr Univ, Coll Marine Sci & Engn, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Dongxue,Zhou, Shun,Yang, Hongsheng. Carbohydrate and amino acids metabolic response to heat stress in the intestine of the sea cucumber Apostichopus japonicus[J]. AQUACULTURE RESEARCH,2017,48(12):5883-5891. |
| APA | Xu, Dongxue,Zhou, Shun,&Yang, Hongsheng.(2017).Carbohydrate and amino acids metabolic response to heat stress in the intestine of the sea cucumber Apostichopus japonicus.AQUACULTURE RESEARCH,48(12),5883-5891. |
| MLA | Xu, Dongxue,et al."Carbohydrate and amino acids metabolic response to heat stress in the intestine of the sea cucumber Apostichopus japonicus".AQUACULTURE RESEARCH 48.12(2017):5883-5891. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Carbohydrate and ami（620KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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