Selection of housekeeping genes as internal controls for quantitative RT-PCR analysis of the veined rapa whelk (Rapana venosa)

doi:10.7717/peerj.3398

	Selection of housekeeping genes as internal controls for quantitative RT-PCR analysis of the veined rapa whelk (Rapana venosa)
	Song, Hao 1,2; Dang, Xin 3; He, Yuan-qiu 3; Zhang, Tao1,4 ; Wang, Hai-yan 1,4
	2017-05-31
发表期刊	PEERJ
卷号	5
文章类型	Article
摘要	Background. The veined rapa whelk Rapana venosa is an important commercial shellfish in China and quantitative real-time PCR (qRT-PCR) has become the standard method to study gene expression in R. venosa. For accurate and reliable gene expression results, qRT-PCR assays require housekeeping genes as internal controls, which display highly uniform expression in different tissues or stages of development. However, to date no studies have validated housekeeping genes in R. venosa for use as internal controls for qRT-PCR.
关键词	Internal Control Real-time Pcr Tissue Development Rapana Venosa
DOI	10.7717/peerj.3398
收录类别	SCI
语种	英语
WOS记录号	WOS:000402560400008
引用统计	被引频次：19[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/137105
专题	海洋生态与环境科学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Ocean Univ China, Coll Fisheries, Qingdao, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Song, Hao,Dang, Xin,He, Yuan-qiu,et al. Selection of housekeeping genes as internal controls for quantitative RT-PCR analysis of the veined rapa whelk (Rapana venosa)[J]. PEERJ,2017,5.
APA	Song, Hao,Dang, Xin,He, Yuan-qiu,Zhang, Tao,&Wang, Hai-yan.(2017).Selection of housekeeping genes as internal controls for quantitative RT-PCR analysis of the veined rapa whelk (Rapana venosa).PEERJ,5.
MLA	Song, Hao,et al."Selection of housekeeping genes as internal controls for quantitative RT-PCR analysis of the veined rapa whelk (Rapana venosa)".PEERJ 5(2017).

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