Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Pumilacidin-Like Lipopeptides Derived from Marine Bacterium Bacillus sp Strain 176 Suppress the Motility of Vibrio alginolyticus | |
Xiu, Pengyuan1,2,3 ; Liu, Rui1,2; Zhang, Dechao4; Sun, Chaomin1,2
| |
| 2017-06-01 | |
| 发表期刊 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY
 |
| 卷号 | 83期号:12 |
| 文章类型 | Article |
| 摘要 | Bacterial motility is a crucial factor during the invasion and colonization processes of pathogens, which makes it an attractive therapeutic drug target. Here, we isolated a marine bacterium (Vibrio alginolyticus strain 178) from a seamount in the tropical West Pacific that exhibits vigorous motility on agar plates and severe pathogenicity to zebrafish. We found that V. alginolyticus 178 motility was significantly suppressed by another marine bacterium, Bacillus sp. strain 176, isolated from the same niche. We isolated, purified, and characterized two different cyclic lipopeptides (CLPs) from Bacillus sp. 176 using high-performance liquid chromatography, mass spectrometry, and nuclear magnetic resonance spectroscopy. The two related CLPs have a pumilacidin-like structure and were both effective inhibitors of V. alginolyticus 178 motility. The CLPs differ by only one methylene group in their fatty acid chains. In addition to motility suppression, the CLPs also induced cell aggregation in the medium and reduced adherence of V. alginolyticus 178 to glass substrates. Notably, upon CLP treatment, the expression levels of two V. alginolyticus flagellar assembly genes (flgA and flgP) dropped dramatically. Moreover, the CLPs inhibited biofilm formation in several other strains of pathogenic bacteria without inducing cell death. This study indicates that CLPs from Bacillus sp. 176 show promise as antimicrobial lead compounds targeting bacterial motility and biofilm formation with a low potential for eliciting antibiotic resistance. |
| 关键词 | Lipopeptide Pumilacidin Vibrio Alginolyticus Motility Antibiotic Antimicrobial Antibiofilm |
| DOI | 10.1128/AEM.00450-17 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000402386500018 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/137093 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Dept Marine Organism Taxon & Phylogeny, Qingdao, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Xiu, Pengyuan,Liu, Rui,Zhang, Dechao,et al. Pumilacidin-Like Lipopeptides Derived from Marine Bacterium Bacillus sp Strain 176 Suppress the Motility of Vibrio alginolyticus[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2017,83(12). |
| APA | Xiu, Pengyuan,Liu, Rui,Zhang, Dechao,&Sun, Chaomin.(2017).Pumilacidin-Like Lipopeptides Derived from Marine Bacterium Bacillus sp Strain 176 Suppress the Motility of Vibrio alginolyticus.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,83(12). |
| MLA | Xiu, Pengyuan,et al."Pumilacidin-Like Lipopeptides Derived from Marine Bacterium Bacillus sp Strain 176 Suppress the Motility of Vibrio alginolyticus".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 83.12(2017). |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Pumilacidin-Like Lip（2690KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论