Interaction between granulin A and enolase 1 attenuates the migration and invasion of human hepatoma cells

doi:10.18632/oncotarget.16328

IOCAS-IR > 实验海洋生物学重点实验室

	Interaction between granulin A and enolase 1 attenuates the migration and invasion of human hepatoma cells
	Chen, Xiaoliang 1; Xu, Huanli 1; Wu, Ning 2; Liu, Xiujun 3; Qiao, Gan 1; Su, Shuonan 1; Tian, Ye 1; Yuan, Ru 1; Li, Cong 1; Liu, Xiaohui 1; Lin, Xiukun 1
	2017-05-02
发表期刊	ONCOTARGET
卷号	8 期号:18 页码:30305-30316
文章类型	Article
摘要	Granulin A (GRN A), a peptide with a molecular 6 kDa, is derived from proteolysis of progranulin (PGRN). Previous study in our laboratory has shown that GRN A is able to inhibit cancer cell growth significantly. In the present study, we confirmed that GRN A can bind to alpha-enolase (ENO1) specifically as analyzed using Pull-down/MS approaches. The interaction of GRN A with ENO1 was further confirmed by Western blotting and Surface plasmon resonance (SPR) analysis. Treatment of human HepG-2 cells with GRN A inhibited cancer cell growth as well as migration and invasion of cancer cells as analyzed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazoliumbromide (MTT) and Scratch wound healing assay as well as Transwell experiments. Additionally, GRN A treatment results in augmentation of glucose uptake in cancer cells. Further study reveals that higher expression of ENO1 reversed the inhibitory effects of GRN A on migration and invasion of HepG-2 cells. The increase of glucose uptake, as well as the expression of apoptosis-related genes, is also reversed in cells overexpressing ENO1. The study provides solid evidence that there is the interaction between GRN A and ENO1 and the interaction is responsible for the effects of GRN A on glucose uptake as well as cancer cell migration and invasion.
关键词	Enolase 1 Granulin a Invasion Migration Protein-protein Interaction
DOI	10.18632/oncotarget.16328
收录类别	SCI
语种	英语
WOS记录号	WOS:000400456200061
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/137063
专题	实验海洋生物学重点实验室
作者单位	1.Capital Med Univ, Dept Pharmacol, Beijing 100069, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266003, Peoples R China 3.Chinese Acad Med Sci, Inst Med Biotechnol, Beijing 100050, Peoples R China
推荐引用方式 GB/T 7714	Chen, Xiaoliang,Xu, Huanli,Wu, Ning,et al. Interaction between granulin A and enolase 1 attenuates the migration and invasion of human hepatoma cells[J]. ONCOTARGET,2017,8(18):30305-30316.
APA	Chen, Xiaoliang.,Xu, Huanli.,Wu, Ning.,Liu, Xiujun.,Qiao, Gan.,...&Lin, Xiukun.(2017).Interaction between granulin A and enolase 1 attenuates the migration and invasion of human hepatoma cells.ONCOTARGET,8(18),30305-30316.
MLA	Chen, Xiaoliang,et al."Interaction between granulin A and enolase 1 attenuates the migration and invasion of human hepatoma cells".ONCOTARGET 8.18(2017):30305-30316.

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