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| Transcriptome analysis on the exoskeleton formation in early developmetal stages and reconstruction scenario in growth-moulting in Litopenaeus vannamei | |
Gao, Yi1; Wei, Jiankai3 ; Yuan, Jianbo1; Zhang, Xiaojun1,2; Li, Fuhua1,2; Xiang, Jianhai1,2
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| 2017-04-24 | |
| 发表期刊 | SCIENTIFIC REPORTS
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| 卷号 | 7 |
| 文章类型 | Article |
| 摘要 | Exoskeleton construction is an important issue in shrimp. To better understand the molecular mechanism of exoskeleton formation, development and reconstruction, the transcriptome of the entire developmental process in Litopenaeus vannamei, including nine early developmental stages and eight adult-moulting stages, was sequenced and analysed using Illumina RNA-seq technology. A total of 117,539 unigenes were obtained, with 41.2% unigenes predicting the full-length coding sequence. Gene Ontology, Clusters of Orthologous Group ( COG), the Kyoto Encyclopedia of Genes and Genomes (KEGG)analysis and functional annotation of all unigenes gave a better understanding of the exoskeleton developmental process in L. vannamei. As a result, more than six hundred unigenes related to exoskeleton development were identified both in the early developmental stages and adult- moulting. A cascade of sequential expression events of exoskeleton-related genes were summarized, including exoskeleton formation, regulation, synthesis, degradation, mineral absorption/reabsorption, calcification and hardening. This new insight on major transcriptional events provide a deep understanding for exoskeleton formation and reconstruction in L. vannamei. In conclusion, this is the first study that characterized the integrated transcriptomic profiles cover the entire exoskeleton development from zygote to adult- moulting in a crustacean, and these findings will serve as significant references for exoskeleton developmental biology and aquaculture research. |
| DOI | 10.1038/s41598-017-01220-6 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000400048600001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/137046 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Ocean Univ China, Qingdao 266071, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Gao, Yi,Wei, Jiankai,Yuan, Jianbo,et al. Transcriptome analysis on the exoskeleton formation in early developmetal stages and reconstruction scenario in growth-moulting in Litopenaeus vannamei[J]. SCIENTIFIC REPORTS,2017,7. |
| APA | Gao, Yi,Wei, Jiankai,Yuan, Jianbo,Zhang, Xiaojun,Li, Fuhua,&Xiang, Jianhai.(2017).Transcriptome analysis on the exoskeleton formation in early developmetal stages and reconstruction scenario in growth-moulting in Litopenaeus vannamei.SCIENTIFIC REPORTS,7. |
| MLA | Gao, Yi,et al."Transcriptome analysis on the exoskeleton formation in early developmetal stages and reconstruction scenario in growth-moulting in Litopenaeus vannamei".SCIENTIFIC REPORTS 7(2017). |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Transcriptome analys（4296KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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