The biofilteration ability of oysters (Crassostrea gigas) to reduce Aeromonas salmonicida in salmon culture

doi:10.1007/s00253-017-8296-3

	The biofilteration ability of oysters (Crassostrea gigas) to reduce Aeromonas salmonicida in salmon culture
	Ma, Xiaona 1,2; Li, Xian 1; Sun, Guoxiang 1; Sharawy, Zaki Zaki 1,3; Qiu, Tianlong1 ; Du, Yishuai 1; Liu, Ying4
	2017-07-01
发表期刊	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷号	101 期号:14 页码:5869-5880
文章类型	Article
摘要	Pathogen contamination in the environment is inevitable with the rapid development of intensive aquaculture. Therefore, alternative ecofriendly biological strategies to control pathogenic bacteria are required. However, our aim was to investigate the ability of oysters (Crassostrea gigas) to filter the important opportunistic pathogen, Aeromonas salmonicida (strain C4), using a green fluorescent protein tag (GFP) in the Atlantic salmon (Salmo salar) farming wastewater. Hence, A. salmonicida removal efficiency and ingestion rate were detected in two different oyster stages (larvae and adults). To evaluate the practical performance of oysters as A. salmonicida biofilter, adult oysters were applied to an integrated constructed wetlands system (ICWS) and their long-term C4-GFP removal efficiency was recorded for 60 days. Overall, our results clearly indicated that oysters had substantial A. salmonicida removal ability via their ingestion process when observed under a fluorescent microscope. Approximately 88-95% of C4-GFP was removed by oyster larvae at an ingestion rate of 6.4 x 10(3)-6.2 x 10(5) CFU/h center dot ind, while 79-92% of C4-GFP was removed by adult oysters at an ingestion rate of 2.1 x 10(4)-3.1 x 10(6) CFU/h center dot ind. Furthermore, 57.9 +/- 17.2% of C4-GFP removal efficiency was achieved when oysters were applied to ICWS. We, therefore, concluded that using oysters as a biofilter represents an effective alternative for removing A. salmonicida from aquaculture wastewater. However, the fate of oysters after ingesting the pathogenic bacteria, acting as a potential reservoir or vector for pathogens, is still debatable. This research provides the basis for the application of oysters as a biofilter to remove pathogens from aquaculture wastewater in industrialized production.
关键词	Bacterial Control Aeromonas Salmonicida Oysters Biofilter Wastewater Treatments
DOI	10.1007/s00253-017-8296-3
收录类别	SCI
语种	英语
WOS记录号	WOS:000405181100026
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/137033
专题	海洋生态与环境科学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.NIOF, Aquaculture Div, Suez, Egypt 4.Dalian Ocean Univ, Marine Sci & Environm Engn Sch, Dalian 116023, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Ma, Xiaona,Li, Xian,Sun, Guoxiang,et al. The biofilteration ability of oysters (Crassostrea gigas) to reduce Aeromonas salmonicida in salmon culture[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2017,101(14):5869-5880.
APA	Ma, Xiaona.,Li, Xian.,Sun, Guoxiang.,Sharawy, Zaki Zaki.,Qiu, Tianlong.,...&Liu, Ying.(2017).The biofilteration ability of oysters (Crassostrea gigas) to reduce Aeromonas salmonicida in salmon culture.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,101(14),5869-5880.
MLA	Ma, Xiaona,et al."The biofilteration ability of oysters (Crassostrea gigas) to reduce Aeromonas salmonicida in salmon culture".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 101.14(2017):5869-5880.

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