Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Identification and function analysis of an anti-lipopolysaccharide factor from the ridgetail prawn Exopalaemon carinicauda | |
Lv, Xinjia1,2 ; Li, Shihao1,3; Liu, Fei1; Li, Fuhua1,3,4; Xiang, Jianhai1,3
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| 2017-05-01 | |
| 发表期刊 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
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| 卷号 | 70页码:128-134 |
| 文章类型 | Article |
| 摘要 | Anti-lipopolysaccharide factor is a kind of antimicrobial peptide (AMP) with broad-spectrum activities against bacteria, virus, and fungi in crustacean. Different isoforms of ALFs showed different activities to virus or bacteria. Therefore, discovery of more ALFs will provide new insights into drug development and disease control. Here we reported an ALF gene, EcALF1, isolated from the ridgetail prawn Exopalaemon carinicauda. Its transcripts was mainly detected in hemocytes. Silencing of EcALF1 caused a lesion of hepatopancreas and finally led to death of the prawn. In vivo bacteria detection to the hepatopancreas showed that the colonies of thiosulphate citrate bile salts (TCBS) cultured bacteria in EcALF1-silenced prawn increased dramatically. We identified the dominant bacteria cultured in TCBS and found that Vibrio alginolyticus and Vibrio parahaemolyticus were dominant bacteria which were pathogenic species to prawn. We further synthesized the functional domain, LPS binding domain (LBD) of EcALF1, and its antibacterial and antiviral activities were detected. The synthetic EcLBD1 peptide showed very strong antibacterial activity against Micro coccus luteus and V. alginolyticus. Pre-incubation of WSSV with EcLBD1 peptide reduced the pathogenicity of WSSV infection to the prawn. The present data showed the important roles of an ALF gene in modulating the in vivo bacterial proliferation in E. carinicauda and provided new insight in disease control in aquaculture. (C) 2017 Elsevier Ltd. All rights reserved. |
| 关键词 | Anti-lipopolysaccharide Factor Rna Interference Bacterial Modulation Antibacterial Antiviral |
| DOI | 10.1016/j.dci.2017.01.010 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000394635500014 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/136796 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China 4.Natl & Local Joint Engn Lab Ecol Mariculture, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Lv, Xinjia,Li, Shihao,Liu, Fei,et al. Identification and function analysis of an anti-lipopolysaccharide factor from the ridgetail prawn Exopalaemon carinicauda[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,70:128-134. |
| APA | Lv, Xinjia,Li, Shihao,Liu, Fei,Li, Fuhua,&Xiang, Jianhai.(2017).Identification and function analysis of an anti-lipopolysaccharide factor from the ridgetail prawn Exopalaemon carinicauda.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,70,128-134. |
| MLA | Lv, Xinjia,et al."Identification and function analysis of an anti-lipopolysaccharide factor from the ridgetail prawn Exopalaemon carinicauda".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 70(2017):128-134. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Identification and f（1289KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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