Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Optimizing production of asperolide A, a potential anti-tumor tetranorditerpenoid originally produced by the algal-derived endophytic fungus Aspergillus wentii EN-48 | |
Xu Rui1,2 ; Li Xiaoming1; Xu Gangming1; Wang Bingui1
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| 2017-05-01 | |
| 发表期刊 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| 卷号 | 35期号:3页码:658-663 |
| 文章类型 | Article |
| 摘要 | The marine algal-derived endophytic fungus Aspergillus wentii EN-48 produces the potential anti-tumor agent asperolide A, a tetranorlabdane diterpenoid active against lung cancer. However, the fermentation yield of asperolide A was very low and only produced in static cultures. Static fermentation conditions of A. wentii EN-48 were optimized employing response surface methodology to enhance the production of asperolide A. The optimized conditions resulted in a 13.9-fold yield enhancement, which matched the predicted value, and the optimized conditions were successfully used in scale-up fermentation for the production of asperolide A. Exogenous addition of plant hormones (especially 10 mu mol/L methyl jasmonate) stimulated asperolide A production. To our knowledge, this is first optimized production of an asperolide by a marine-derived fungus. The optimization is Effective and valuable to supply material for further anti-tumor mechanism studies and preclinical evaluation of asperolide A and other norditerpenoids. |
| 关键词 | Aspergillus Wentii Asperolide a Anti-tumor Agent Fermentation Optimization |
| DOI | 10.1007/s00343-017-6028-2 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000399168400020 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/136720 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol,Lab Marine Biol & Biotec, Qingdao Natl Lab Marine Sci & Technol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Xu Rui,Li Xiaoming,Xu Gangming,et al. Optimizing production of asperolide A, a potential anti-tumor tetranorditerpenoid originally produced by the algal-derived endophytic fungus Aspergillus wentii EN-48[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2017,35(3):658-663. |
| APA | Xu Rui,Li Xiaoming,Xu Gangming,&Wang Bingui.(2017).Optimizing production of asperolide A, a potential anti-tumor tetranorditerpenoid originally produced by the algal-derived endophytic fungus Aspergillus wentii EN-48.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,35(3),658-663. |
| MLA | Xu Rui,et al."Optimizing production of asperolide A, a potential anti-tumor tetranorditerpenoid originally produced by the algal-derived endophytic fungus Aspergillus wentii EN-48".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 35.3(2017):658-663. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Optimizing productio（742KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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