Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Assessment of housekeeping genes as internal references in quantitative expression analysis during early development of oyster | |
Huan, Pin1 ; Wang, Hongxia1; Liu, Baozhong1,2
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| 2016-10-01 | |
| 发表期刊 | GENES & GENETIC SYSTEMS
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| 卷号 | 91期号:5页码:257-265 |
| 文章类型 | Article |
| 摘要 | The early development of mollusks exhibits important characteristics from the developmental and evolutionary perspective. With the increasing number of genome-wide studies, accurate analyses of quantitative gene expression during development are impeded by the lack of validated reference genes. To improve the situation, in this study, we analyzed the expression stability of seven candidate housekeeping genes during early development of the Pacific oyster Crassostrea gigas: actin, glyceraldehyde-3-phosphate dehydrogenase (gapdh), a subunit of elongation factor 1 (elf1 alpha), adp-ribosylation factor 1 (arf1), heterogeneous nuclear ribonucleoprotein q, ubiquitin-conjugating enzyme e2d2 and ribosomal protein s18. We focused on 11 stages from oocyte to D-veliger, which include crucial developmental processes such as axis determination, gastrulation and shell formation. Gene expression stabilities were assessed with the three commonly used programs geNorm, NorniFinder and BestKeeper. Although the results obtained with the three programs varied to some extent, in general, arf1, elf1 alpha and gapdh were highly ranked and actin was poorly ranked. This analysis also indicated that multiple genes should be used for normalization, and we concluded that arf1-elf1 alpha-gapdh should be used as internal references. The findings of this study will help researchers to obtain accurate results in future quantitative gene expression analysis of development in bivalve mollusks. |
| 关键词 | Crassostrea Gigas Expression Stability Internal Reference Normalization Real-time Pcr |
| DOI | 10.1266/ggs.16-00007 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000399356100002 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/136709 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 1 Wenhai Rd, Qingdao 266000, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Huan, Pin,Wang, Hongxia,Liu, Baozhong. Assessment of housekeeping genes as internal references in quantitative expression analysis during early development of oyster[J]. GENES & GENETIC SYSTEMS,2016,91(5):257-265. |
| APA | Huan, Pin,Wang, Hongxia,&Liu, Baozhong.(2016).Assessment of housekeeping genes as internal references in quantitative expression analysis during early development of oyster.GENES & GENETIC SYSTEMS,91(5),257-265. |
| MLA | Huan, Pin,et al."Assessment of housekeeping genes as internal references in quantitative expression analysis during early development of oyster".GENES & GENETIC SYSTEMS 91.5(2016):257-265. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Assessment of housek（682KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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