Lectin functionalized ZnO nanoarrays as a 3D nano-biointerface for bacterial detection

doi:10.1016/j.talanta.2017.03.007

	Lectin functionalized ZnO nanoarrays as a 3D nano-biointerface for bacterial detection
	Zheng, Laibao1,2 ; Wan, Yi 1; Qi, Peng1 ; Sun, Yan 1; Zhang, Dun 1; Yu, Liangmin 3,4
	2017-05-15
发表期刊	TALANTA
卷号	167 页码:600-606
文章类型	Article
摘要	The detection of pathogenic bacteria is essential in various fields, such as food safety, water environmental analysis, or clinical diagnosis. Although rapid and selective techniques have been achieved based on the fast and specific binding of recognitions elements and target, the sensitive detection of bacterial pathogens was limited by their low targets-binding efficiency. The three-dimensional (3D) nano-biointerface, compared with the two-dimensional (2D) flat substrate, has a much higher binding capacity, which can offer more reactive sites to bind with bacterial targets, resulting in a great improvement of detection sensitivity. Herein, a lectin functionalized ZnO nanorod (ZnO-NR) array has been fabricated and employed as a 3D nano-biointerface for Escherichia coli (E. con) capture and detection by multivalent binding of concanavalin A (ConA) with polysaccharides on the cellular surface of E. coli. The 3D lectin functionalized ZnO-NR array-based assay shows reasonable detection limit and efficiently expanded linear range (1.0x10(3) to 1.0x10(7) cfu mL(-1)) for pathogen detection. The platform has a potential for further applications and provides an excellent sensitivity approach for detection of pathogenic bacteria.
关键词	3d Nano-interface Zno-nr Arrays Lectin Functionalization Bacterial Detection
DOI	10.1016/j.talanta.2017.03.007
收录类别	SCI
语种	英语
WOS记录号	WOS:000399511600075
引用统计	被引频次：35[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/136699
专题	海洋环境腐蚀与生物污损重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Ocean Univ China, Key Lab Marine Chem Theory & Technol, Minist Educ, Qingdao 266100, Peoples R China 4.Ocean Univ China, Qingdao Collaborat Innovat Ctr Marine Sci & Techn, Qingdao 266100, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zheng, Laibao,Wan, Yi,Qi, Peng,et al. Lectin functionalized ZnO nanoarrays as a 3D nano-biointerface for bacterial detection[J]. TALANTA,2017,167:600-606.
APA	Zheng, Laibao,Wan, Yi,Qi, Peng,Sun, Yan,Zhang, Dun,&Yu, Liangmin.(2017).Lectin functionalized ZnO nanoarrays as a 3D nano-biointerface for bacterial detection.TALANTA,167,600-606.
MLA	Zheng, Laibao,et al."Lectin functionalized ZnO nanoarrays as a 3D nano-biointerface for bacterial detection".TALANTA 167(2017):600-606.

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Lectin functionalize（1248KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览