Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Isolation, characterization, and identification of proteins interfering with enzyme-linked immunosorbent assay of antibiotics in fish matrix | |
Wang, Xiudan1,2,3 ; Lin, Hong1; Cao, Limin1; Zheng, Hongwei1; Cui, Mengqi1; Du, Shuyuan1; Sui, Jianxin1
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| 2016-10-01 | |
| 发表期刊 | FOOD SCIENCE AND BIOTECHNOLOGY
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| 卷号 | 25期号:5页码:1265-1273 |
| 文章类型 | Article |
| 摘要 | The interaction between some proteins and immune globulin has been confirmed as an important source of matrix interference with the immunoassay of fishery products, but detailed biochemical properties of these proteins have not been indicated. Two interference-inducing proteins (42 and 36 kD) in flounder were isolated, characterized, and identified. Their influences on the immunoassay of norfloxacin were confirmed by western blotting and enzyme-linked immunosorbent assay. The pI value and pH stability of the two proteins were also investigated. Using LC-MS/MS, the two proteins were identified as fructose-bisphosphate aldolase, and such results were partly verified by the aldolase activity of the 42 and 36 kD isolates. Considering the prevalence of these proteins (as multi-functional aldolase of muscles) in foods, these results would help to further understand the matrix effects in various immunoassays as well as the development of effective techniques to improve the efficiency of immunoassays. |
| 关键词 | Matrix Interference Fish Protein Enzyme-linked Immunosorbent Assay Biochemical Property Aldolase |
| DOI | 10.1007/s10068-016-0200-x |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000387529400006 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/136210 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Ocean Univ China, Food Safety Lab, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xiudan,Lin, Hong,Cao, Limin,et al. Isolation, characterization, and identification of proteins interfering with enzyme-linked immunosorbent assay of antibiotics in fish matrix[J]. FOOD SCIENCE AND BIOTECHNOLOGY,2016,25(5):1265-1273. |
| APA | Wang, Xiudan.,Lin, Hong.,Cao, Limin.,Zheng, Hongwei.,Cui, Mengqi.,...&Sui, Jianxin.(2016).Isolation, characterization, and identification of proteins interfering with enzyme-linked immunosorbent assay of antibiotics in fish matrix.FOOD SCIENCE AND BIOTECHNOLOGY,25(5),1265-1273. |
| MLA | Wang, Xiudan,et al."Isolation, characterization, and identification of proteins interfering with enzyme-linked immunosorbent assay of antibiotics in fish matrix".FOOD SCIENCE AND BIOTECHNOLOGY 25.5(2016):1265-1273. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Isolation, character（6986KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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