An oyster species-specific miRNA scaffold42648_5080 modulates haemocyte migration by targeting integrin pathway

doi:10.1016/j.fsi.2016.08.032

IOCAS-IR > 实验海洋生物学重点实验室

	An oyster species-specific miRNA scaffold42648_5080 modulates haemocyte migration by targeting integrin pathway
	Chen, Hao1,3 ; Wang, Hao 1; Jiang, Shuai 1; Xu, Jiachao1,3 ; Wang, Lingling 2; Qiu, Limei 1; Song, Linsheng 2
	2016-10-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
卷号	57 页码:160-169
文章类型	Article
摘要	miRNAs are important gene regulators at post-transcriptional level and can modulate diverse biological processes, including immune response. Dozens of species-specific miRNAs have been identified in oyster Crassostrea gigas while their functions remain largely unknown. In the present study, an oyster species specific miRNA scaffold42648_5080 was found responsive to LPS stimulation and might target a total of 31 oyster genes possibly involved in cell communication, cellular localization and cellular response to stimulus. Besides, in gain-of-function assay of scaffold42648_5080 in vivo, the phagocytosis (30.90% in miRNA group verse 23.20% in miRNA control group), apoptosis (3.10% in miRNA group verse 5.30% in miRNA control group) and migration rate (13.88% in miRNA group verse 21.03% in miRNA control group) of oyster haemocytes were found significantly altered after the injection of scaffold42648_5080 mimics. Among the target genes, integrin-linked kinase (CgILK) was considered crucial in cell migration and its interaction with scaffold42648_5080 was then verified both in vitro and in vivo. Consequently, a significant decrease of relative luciferase ratio was observed in CgILK 3'-UTR luciferase reporter assay after transfection of scaffold42648_5080 mimics (0.70-fold of that in blank group, p < 0.01). Meanwhile, when scaffold42648_5080 was overexpressed in vivo (5.41-fold of miRNA control group, p < 0.01), the expression of CgILK declined significantly to 0.25-fold of miRNA control group (p < 0.01). Comparatively, a significant decrease of the haemocyte migration rate (19.76% verse 34.82% in siEGFP control group, p < 0.01) was observed after knock-down of CgILK in vivo. The present study, as far as we know, for the first time revealed the immunomodulation role of an oyster species-specific miRNA, which might provide new insights into miRNA-mediated adaptation mechanism of oysters. (C) 2016 Elsevier Ltd. All rights reserved.
关键词	Species-specific Mirna Cellular Immune Response Integrin-linked Kinase Integrin Pathway Cell Migration
DOI	10.1016/j.fsi.2016.08.032
收录类别	SCI
语种	英语
WOS记录号	WOS:000385327100017
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/136154
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chi, Minist Agr, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Chen, Hao,Wang, Hao,Jiang, Shuai,et al. An oyster species-specific miRNA scaffold42648_5080 modulates haemocyte migration by targeting integrin pathway[J]. FISH & SHELLFISH IMMUNOLOGY,2016,57:160-169.
APA	Chen, Hao.,Wang, Hao.,Jiang, Shuai.,Xu, Jiachao.,Wang, Lingling.,...&Song, Linsheng.(2016).An oyster species-specific miRNA scaffold42648_5080 modulates haemocyte migration by targeting integrin pathway.FISH & SHELLFISH IMMUNOLOGY,57,160-169.
MLA	Chen, Hao,et al."An oyster species-specific miRNA scaffold42648_5080 modulates haemocyte migration by targeting integrin pathway".FISH & SHELLFISH IMMUNOLOGY 57(2016):160-169.

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An oyster species-sp（2059KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览