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| A CgIFNLP receptor from Crassostrea gigas and its activation of the related genes in human JAK/STAT signaling pathway | |
Zhang, Ran1,4; Liu, Rui1; Xin, Lusheng1,3 ; Chen, Hao1,3; Li, Chenghua4; Wang, Lingling2; Song, Linsheng2
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| 2016-12-01 | |
| 发表期刊 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
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| 卷号 | 65页码:98-106 |
| 文章类型 | Article |
| 摘要 | Interferon is a highly pleiotropic cytokine, once binding to its receptors, can activate JAK kinases and STAT transcription factors to initiate the transcription of genes downstream from interferon-stimulated response elements. In the present study, a cytokine receptor-like 3 molecule was selected and cloned from oyster Crassostrea gigas, which contained a fibronectin type III domain (designed CgIFNR-3). The expression pattern of CgIFNR-3 mRNA was detected in all the tested tissues including mantle, gills, hepatopancreas, muscle, and hemocytes, with the highest expression level in hemocytes. After poly (I: C) stimulation, the expression level of CgIFNR-3 in hemocytes was observed to significantly increase at 3 h (13.06-fold, p < 0.01). CgIFNR-3 was indicated to interact with CgIFNLP by in vitro GST pull-down assay, and to activate the expression of transcription factors including ISRE, STAT3 and GAS, in human Janus kinase signal transducer and activator of transcription (JAK/STAT) pathway after co-transfection in HEK-293T cells in the reporter luciferase activity assay. These results suggested that CgIFNR-3 could bind to CgIFNLP as an interferon receptor and participate in the activation of JAK/STAT pathway in human, which will benefit for intensive studies of interferon signaling pathway in mollusc. (C) 2016 Elsevier Ltd. All rights reserved. |
| 关键词 | Crassostrea Gigas Interferon Interferon Receptor Poly (i: c) Interferon Stimulated Genes |
| DOI | 10.1016/j.dci.2016.06.010 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000384388400011 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/135895 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhang, Ran,Liu, Rui,Xin, Lusheng,et al. A CgIFNLP receptor from Crassostrea gigas and its activation of the related genes in human JAK/STAT signaling pathway[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,65:98-106. |
| APA | Zhang, Ran.,Liu, Rui.,Xin, Lusheng.,Chen, Hao.,Li, Chenghua.,...&Song, Linsheng.(2016).A CgIFNLP receptor from Crassostrea gigas and its activation of the related genes in human JAK/STAT signaling pathway.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,65,98-106. |
| MLA | Zhang, Ran,et al."A CgIFNLP receptor from Crassostrea gigas and its activation of the related genes in human JAK/STAT signaling pathway".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 65(2016):98-106. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| A CgIFNLP receptor f（2992KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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