Understanding mechanism of sea cucumber Apostichopus japonicus aestivation: Insights from TMT-based proteomic study

doi:10.1016/j.cbd.2016.06.005

IOCAS-IR > 实验海洋生物学重点实验室

	Understanding mechanism of sea cucumber Apostichopus japonicus aestivation: Insights from TMT-based proteomic study
	Chen, Muyan 1; Li, Xingke 1; Zhu, Aijun 1; Storey, Kenneth B.2; Sun, Lina 3; Gao, Tianxiang 4; Wang, Tianming 5
	2016-09-01
发表期刊	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
卷号	19 页码:78-89
文章类型	Article
摘要	Marine invertebrate aestivation is a unique strategy for summer survival in response to hot marine conditions. The sea cucumber, Apostichopus japonicus, is an excellent model marine invertebrate for studies of environmentally-induced aestivation. In the present study, we used a tandem mass tag (TMT)-coupled LC-MS/MS approach to identify and quantify the global proteome expression profile over the aestivation-arousal cycle of A. japonicus. A total of 3920 proteins were identified from the intestine of sea cucumber. Among them, 630 proteins showed significant differential expression when comparing three conditions of sea cucumbers: non aestivating (active), deep-aestivation (at least 15 days of continuous aestivation), and arousal after aestivation (renewed moving and feeding). Sea cucumbers in deep aestivation showed substantial differentially expressed proteins (143 up-regulated and 267 down-regulated proteins compared with non-aestivating controls). These differentially expressed proteins suggested that protein and phospholipid probably are major fuel sources during hypometabolism and a general attenuation of carbohydrate metabolism was observed during deep aestivation. Differentially expressed proteins also provided the first global picture of a shift in protein synthesis, protein folding, DNA binding, apoptosis, cellular transport and signaling, and cytoskeletal proteins during deep aestivation in sea cucumbers. A comparison of arousal from aestivation with deep aestivation, revealed a general reversal of the changes that occurred in aestivation for most proteins. Western blot detection further validated the significant up-regulation of HSP70 and down-regulation of methyltransferase-like protein 7A-like in deep-aestivation. Our results suggest that there is substantial post-transcriptional regulation of proteins during the aestivation arousal cycle in sea cucumbers. (C) 2016 Elsevier Inc. All rights reserved.
关键词	Aestivation Sea Cucumber Proteome Tandem Mass Tag Post-transcriptional Regulation
DOI	10.1016/j.cbd.2016.06.005
收录类别	SCI
语种	英语
WOS记录号	WOS:000382416300009
引用统计	被引频次：20[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/135755
专题	实验海洋生物学重点实验室
作者单位	1.Ocean Univ China, Coll Fisheries, Qingdao, Peoples R China 2.Carleton Univ, Inst Biochem, 1125 Colonel Dr, Ottawa, ON K1S 5B6, Canada 3.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 4.Zhejiang Ocean Univ, Fishery Coll, Zhoushan, Peoples R China 5.Zhejiang Ocean Univ, Marine Sci & Technol Coll, Zhoushan, Peoples R China
推荐引用方式 GB/T 7714	Chen, Muyan,Li, Xingke,Zhu, Aijun,et al. Understanding mechanism of sea cucumber Apostichopus japonicus aestivation: Insights from TMT-based proteomic study[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2016,19:78-89.
APA	Chen, Muyan.,Li, Xingke.,Zhu, Aijun.,Storey, Kenneth B..,Sun, Lina.,...&Wang, Tianming.(2016).Understanding mechanism of sea cucumber Apostichopus japonicus aestivation: Insights from TMT-based proteomic study.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,19,78-89.
MLA	Chen, Muyan,et al."Understanding mechanism of sea cucumber Apostichopus japonicus aestivation: Insights from TMT-based proteomic study".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 19(2016):78-89.

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