皮状丝孢酵母发酵大米草水解液生产微生物油脂

IOCAS-IR > 海洋环流与波动重点实验室

	皮状丝孢酵母发酵大米草水解液生产微生物油脂
其他题名	Fermentation of Spartina anglica Acid Hydrolysate by Trichosporon cutaneum for Microbial Lipid Production
	沈珺珺
学位类型	博士
	2007-05-29
学位授予单位	中国科学院海洋研究所
学位授予地点	海洋研究所
关键词	皮状丝孢酵母大米草水解发酵微生物油脂
摘要	大米草是一种外来引种植物，本来引进的目的是为了保滩护岸、促淤造陆。然而大米草过度繁殖，侵袭海洋沿岸滩涂，所以综合开发大米草有着重要的经济意义和生态意义。本实验研究着力于将大米草水解液通过皮状丝孢酵母发酵而制取生物油脂。并且随着近年来石油价格的攀升和对于汽车尾气对环境造成的日趋严重的污染，生物柴油的开发受到高度重视。微生物油脂则是生物柴油生产的很好的原料，因为它无需占用耕地，而能够把农业加工剩余物或是其它的含纤维素的生物质转化为油脂。本实验采用产油酵母菌皮状丝孢酵母（Trichosporon cutaneum）直接发酵经过简单脱毒处理的大米草水解液生产微生物油脂。在122℃，固—液比为10%（w/v），分别用1%、2%、4%和6%稀硫酸水解大米草，结果表明在6%的酸浓度下水解40 min后还原糖含量最高，达到22.8 g/L。而同时以1%稀硫酸在140℃下水解大米草，2.8h后，水解液中葡萄糖产量达到最高14.2g/L。确定了摇瓶发酵最适工艺条件：摇床转速200 rpm，接种量10%（v/v），装液量为200 mL (1000-mL三角瓶)。在此基础上，确定了水解液最适浓缩倍数和pH值，水解液浓缩倍数为4.0时产油量高达6.0 g/L，产油率为46.3%。本文为高效利用大米草提供了新的途径。关键词:皮状丝孢酵母,大米草,水解,发酵,微生物油脂
其他摘要	The cordgrass, Spartina anglica is an introduced species that help to stabilise sediments and reduce wave erosion of sea defences. But in recent years, it tends to invade sheltered sand and mudflats, at the upper low marsh level. To explore its ecological and economic values, the bioconversion of partially detoxified acid hydrolysate of Spartina anglica to lipid by Trichosporon cutaneum was studied. And also due to the increase in the price of the petroleum and the environmental concerns about pollution coming from the car gases, biodiesel is becoming a developing area of high concern. Microbial oil is a good resourse of biodiesel oil supply, as it virtually needs no extra farmland while conversion of agricultural residues and other lignocellulosic biomass into lipid. In our research, The hydrolysis of Spartina anglica at 122℃, using four concentrations of sulfuric acid (1%, 2%, 4% and 6%) was investigated with a solid-liquid ratio of 10% (/v). Maximal total reducing sugar of 22.8 g/L was realized with 6% sulfuric acid for 40 min. Moreover, at 140℃ using 1% sulfuric acid glucose was 14.2g/L. The optimal fermentation conditions of cell biomass production were as follows: 200 r/min, initial pH 6.0, and an inoculum level of 10% (v/v), 200 mL of medium in 1000-mL Erlenmeyer flask. Moreover, the optimum concentrating factor of hydrolysate was 4.0. Under these conditions, lipid was produced up to 5.9 g/L，lipid content reaches the highest point which was 46.3%. This work provided valuable information for development of an efficient process to produce microbial lipid from Spartina anglica acid hydrolysate. Key words:Trichosporon cutaneum,Spartina anglica,hydrolysate,fermentation,microbial lipid
页数	46
语种	中文
文献类型	学位论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/1341
专题	海洋环流与波动重点实验室
推荐引用方式 GB/T 7714	沈珺珺. 皮状丝孢酵母发酵大米草水解液生产微生物油脂[D]. 海洋研究所. 中国科学院海洋研究所,2007.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
10001_20042800681206（373KB）			限制开放	--	浏览