Invertase-mediated system for simple and rapid detection of pathogen

doi:10.1016/j.snb.2016.04.098

	Invertase-mediated system for simple and rapid detection of pathogen
	Wan, Yi; Qi, Peng; Zeng, Yan; Sun, Yan; Zhang, Dun
	2016-10-05
发表期刊	SENSORS AND ACTUATORS B-CHEMICAL
卷号	233 页码:454-458
文章类型	Article
摘要	Portable, low-cost and quantitative analysis of pathogen at home and in the field remain a key challenge in medical diagnostics and food safety. Despite many years of research, very few such tools are available. Motivated by present clinical requirements, pocket-sized device, such as commercial glucose meter (CGM), have emerged. Taking advantage of the CGM, we develop an approach to quantify bacteria. Molecular recognition elements, such as aptamer, coupled with a signal amplification method based on invertase-promoted hydrolysis of sucrose for the sensitive and selective detection of bacteria. This paper aims to develop a portable device combined with enzyme-mediated enhancement for biological/chemical analyte detection. A linear relationship between the response and the logarithm of the Staphylococcus aureus concentration using this method was obtained for concentrations ranging from 1.2 x 10(5) to 1.2 x 10(8) cfu mL(-1), with a detection limit of 1.0 x 10(5) cfu mL(-1). This study demonstrates an approach for bacteria detection and also provides a powerful platform to monitor bacterial infection, thus rendering potential broad antibacterial applications and bacteria diagnosis. (C) 2016 Elsevier B.V. All rights reserved.
关键词	Commercial Glucose Meter Bacteria Detection Invertase Aptamer
DOI	10.1016/j.snb.2016.04.098
收录类别	SCI
语种	英语
WOS记录号	WOS:000376384100034
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/131084
专题	海洋环境腐蚀与生物污损重点实验室
作者单位	Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wan, Yi,Qi, Peng,Zeng, Yan,et al. Invertase-mediated system for simple and rapid detection of pathogen[J]. SENSORS AND ACTUATORS B-CHEMICAL,2016,233:454-458.
APA	Wan, Yi,Qi, Peng,Zeng, Yan,Sun, Yan,&Zhang, Dun.(2016).Invertase-mediated system for simple and rapid detection of pathogen.SENSORS AND ACTUATORS B-CHEMICAL,233,454-458.
MLA	Wan, Yi,et al."Invertase-mediated system for simple and rapid detection of pathogen".SENSORS AND ACTUATORS B-CHEMICAL 233(2016):454-458.

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