Production of crocetin in transgenic Chlorella vulgaris expressing genes crtRB and ZCD1

doi:10.1007/s10811-015-0730-2

IOCAS-IR > 实验海洋生物学重点实验室

	Production of crocetin in transgenic Chlorella vulgaris expressing genes crtRB and ZCD1
	Lou, Sulin 1; Wang, Liuying 1; He, Lijuan 1; Wang, Zhaokai 1; Wang, Guangce 2,3; Lin, Xiangzhi 1
	2016-06-01
发表期刊	JOURNAL OF APPLIED PHYCOLOGY
卷号	28 期号:3 页码:1657-1665
文章类型	Article
摘要	Crocetin is one of the major active ingredients of the rare medicinal herb saffron (Crocus sativus L.), also known as crocus. Crocetin has a wide range of effects including antitumor, anticancer, antioxidant, and antiatherosclerotic activity. Crocetin is currently only obtained via extraction from crocus stigmas, with limited yields. This study developed a new method of production of crocetin from genetically engineered Chlorella vulgaris, a unicellular, fast-growing, and heterotrophically cultured green microalga. A plant expression vector carrying the genes crtRB and ZCD1, which encode enzymes controlling critical steps in crocetin biosynthesis, was introduced into C. vulgaris using the Agrobacterium tumefaciens-mediated transformation method. After hygromycin screening, resistant C. vulgaris strains were achieved. Polymerase chain reaction amplification confirmed that the crtRB and ZCD1 were successfully integrated into the C. vulgaris genome. High-performance liquid chromatography analysis of methanol extraction showed that the transgenic C. vulgaris was able to produce crocetin, while wild-type C. vulgaris was not. These results strongly suggest that introducing crtRB and ZCD1 into C. vulgaris can successfully produce crocetin. This is a new method of crocetin production in C. vulgaris to compensate for the shortage of crocetin.
关键词	Chlorella Vulgaris Crtrb Zcd1 Genetic Engineering Crocetin
DOI	10.1007/s10811-015-0730-2
收录类别	SCI
语种	英语
WOS记录号	WOS:000375315100021
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/131065
专题	实验海洋生物学重点实验室
作者单位	1.State Ocean Adm, Engn Res Ctr Marine Biol Resource Comprehens Util, Inst Oceanog 3, 184 Daxue Rd, Xiamen 361005, Fujian Province, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Nanhai Rd 7, Qingdao, Shandong, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Lou, Sulin,Wang, Liuying,He, Lijuan,et al. Production of crocetin in transgenic Chlorella vulgaris expressing genes crtRB and ZCD1[J]. JOURNAL OF APPLIED PHYCOLOGY,2016,28(3):1657-1665.
APA	Lou, Sulin,Wang, Liuying,He, Lijuan,Wang, Zhaokai,Wang, Guangce,&Lin, Xiangzhi.(2016).Production of crocetin in transgenic Chlorella vulgaris expressing genes crtRB and ZCD1.JOURNAL OF APPLIED PHYCOLOGY,28(3),1657-1665.
MLA	Lou, Sulin,et al."Production of crocetin in transgenic Chlorella vulgaris expressing genes crtRB and ZCD1".JOURNAL OF APPLIED PHYCOLOGY 28.3(2016):1657-1665.

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