Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Identification of the MmeHairy gene and expression analysis affected by two SNPs in the 3 '-untranslated region in the clam Meretrix meretrix | |
Nie, Qing1,3 ; Yue, Xin1,2; Liu, Baozhong1,2
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| 2016-04-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| 卷号 | 51页码:46-52 |
| 文章类型 | Article |
| 摘要 | As a bHLH transcriptional repressor, Hairy-related proteins can bind to DNA sites in target gene promoters and negatively regulate gene transcription. In the present study, the full-length cDNA of Hairy was obtained from the clam Meretrix meretrix (MmeHairy), and two SNPs in the 3'-untranslated region (UTR) of this gene, SNP1066 and 1067, were identified and characterized. Multiple sequence alignment and phylogenetic analysis revealed that MmeHairy belongs to the Hairy protein subfamily. Analysis of tissue expression patterns showed that the mRNA of MmeHairy had the highest expression level in the hepatopancreas. The expression levels of MmeHairy were up-regulated in the hepatopancreas after Vibrio challenge. Genotyping and quantitative analysis showed that the mRNA levels of MmeHairy were significantly different among individual clams with different genotypes at SNP1066 and 1067 (P < 0.05), which indicated that these two SNP loci may affect the expression of MmeHairy and could be used as candidate markers for future selection in M. meretrix breeding programs. (C) 2016 Elsevier Ltd. All rights reserved. |
| 关键词 | Meretrix Meretrix Hairy Single Nucleotide Polymorphism (Snp) Vibrio-resistance |
| DOI | 10.1016/j.fsi.2016.02.003 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000374617700007 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/131059 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Yancheng Inst Technol, Dept Marine Sci & Technol, Coll Marine & Biol Engn, Yancheng 224051, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Nie, Qing,Yue, Xin,Liu, Baozhong. Identification of the MmeHairy gene and expression analysis affected by two SNPs in the 3 '-untranslated region in the clam Meretrix meretrix[J]. FISH & SHELLFISH IMMUNOLOGY,2016,51:46-52. |
| APA | Nie, Qing,Yue, Xin,&Liu, Baozhong.(2016).Identification of the MmeHairy gene and expression analysis affected by two SNPs in the 3 '-untranslated region in the clam Meretrix meretrix.FISH & SHELLFISH IMMUNOLOGY,51,46-52. |
| MLA | Nie, Qing,et al."Identification of the MmeHairy gene and expression analysis affected by two SNPs in the 3 '-untranslated region in the clam Meretrix meretrix".FISH & SHELLFISH IMMUNOLOGY 51(2016):46-52. |
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