The polymorphisms of a MIF gene and their association with Vibrio resistance in the clam Meretrix meretrix

doi:10.1016/j.dci.2016.04.013

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	The polymorphisms of a MIF gene and their association with Vibrio resistance in the clam Meretrix meretrix
	Zou, Linhu1,2 ; Liu, Baozhong1,3
	2016-09-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷号	62 页码:116-126
文章类型	Article
摘要	Macrophage migration inhibitory factor (MIF) is an important proinflammatory cytokine that mediates both innate and adaptive immune responses. In this study, a homolog of MIF was identified in the clam Meretrix meretrix. Ten SNPs in the DNA partial sequence of MmMIF were found to be significantly associated with Vibrio resistance (P < 0.05). Distinct expression patterns of MmMIF among different haplotypes were observed after Vibrio challenge. The results showed that haplotypes did not affect MmMIF expression in the negative control group, while the expression of MmMIF in clams with Hap1 and Hap1/Hap2 was significantly lower than that with Hap2 at 24 h in the PBS-injected group but significantly higher than that with Hap2 in the Vibrio-injected group. The results also indicate that Hap1 and Hap1/Hap2 can specifically respond to mechanical stimulation while Hap2 can specifically respond to Vibrio infection. The effect of a missense mutation was detected by site-directed mutagenesis using fusion expression of the protein, which showed that the SNP g.737 (I > V) has no effect on redox activity and tautomerase activity. These studies identified a potential marker that is enriched in Vibrio-resistant clams that can be used for the genetic breeding of Meretrix meretrix. (C) 2016 Elsevier Ltd. All rights reserved.
关键词	Meretrix Meretrix Mmmif Vibrio Resistance Snps Haplotype
DOI	10.1016/j.dci.2016.04.013
收录类别	SCI
语种	英语
WOS记录号	WOS:000378456900014
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/130998
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Zou, Linhu,Liu, Baozhong. The polymorphisms of a MIF gene and their association with Vibrio resistance in the clam Meretrix meretrix[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,62:116-126.
APA	Zou, Linhu,&Liu, Baozhong.(2016).The polymorphisms of a MIF gene and their association with Vibrio resistance in the clam Meretrix meretrix.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,62,116-126.
MLA	Zou, Linhu,et al."The polymorphisms of a MIF gene and their association with Vibrio resistance in the clam Meretrix meretrix".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 62(2016):116-126.

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