水中亚铁对大菱鲆幼鱼红细胞核异常及碱性磷酸酶和超氧化物歧化酶活性的影响

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	水中亚铁对大菱鲆幼鱼红细胞核异常及碱性磷酸酶和超氧化物歧化酶活性的影响
	吴志昊; 尤锋; 王英芳; 文爱韵; 马得友; 徐永立; 张培军
	2011
发表期刊	海洋湖沼通报
ISSN	1003-6482
卷号	000 期号:4 页码:45-51
摘要	本文比较研究了Fe(II)浓度为0.01、0.05、0.1、0.5、1、2mg/L及不同处理时间下大菱鲆幼鱼(Scophthalmus maximus)外周血红细胞核异常以及碱性磷酸酶(AKP)和超氧化物岐化酶(SOD)活力的变化。结果表明,在整个实验期间,0.01mg/L处理组的核异常细胞率及总核异常率均无明显变化。但0.05mg/L的Fe(II)可以引起大菱鲆血液红细胞中出现微核、核质外凸、核质内凹、双核等损伤,这种损伤表现出一定的时间累积效应。在0.1mg/L及较高浓度组中,也都观察到核异常现象:处理1天时,2mg/L组大菱鲆红细胞的核异常率及总核异常率明显增高(P<0.05)。处理14...
其他摘要	Groundwater is widely used in the turbot and other fish culture,in which the concentration of Fe（Ⅱ） could be higher under anoxic environment.In this study,nucleus anomaly in erythrocytes and AKP,SOD activities in serum of juvenile turbot Scophthalmus maximus were studied when juvenile turbot was exposed to 0.01 mg l-1,0.05 mg l-1,0.1 mg l-1,0.5 mg l-1,1 mg l-1,and 2mg l-1 waterborne Fe（Ⅱ）.The results showed that the frequencies of micronucleus and nucleus anomaly between the 0.01 mg/L and control groups were not significantly different during the experiment period,and there were no significant differences of the micronucleus frequencies among all the groups.But,micronucleus and nucleus anomaly in erythrocytes of juvenile turbot could be observed when exposed to 0.05mg/L waterborne Fe（Ⅱ）.These phenomena also appeared in the groups of higher waterborne Fe（Ⅱ） concentration.On day 1,no significant difference was observed among the serum AKP activities of turbot in all the groups.The serum SOD activities in the 2mg/L groups were significant lower than which in the blank and ascorbic acid control groups（P0.05）.On day 14,the serum AKP activities in the 0.5mg/L and 1mg/L groups were significant higher than which in the blank control groups（P0.05）.The serum SOD activities in all the experiment groups were not significant different from which in the two control groups.On day 28,the serum AKP and SOD activities showed a dosage-effect curve.The results could help to define the safe concentration of ferrous in turbot culture.
关键词	大菱鲆(Scophthalmus Maximus)幼鱼亚铁fe(Ii) 核异常碱性磷酸酶超氧化物歧化酶
收录类别	CSCD
语种	中文
CSCD记录号	CSCD:4393930
引用统计	被引频次：2[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12993
专题	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	吴志昊,尤锋,王英芳,等. 水中亚铁对大菱鲆幼鱼红细胞核异常及碱性磷酸酶和超氧化物歧化酶活性的影响[J]. 海洋湖沼通报,2011,000(4):45-51.
APA	吴志昊.,尤锋.,王英芳.,文爱韵.,马得友.,...&张培军.(2011).水中亚铁对大菱鲆幼鱼红细胞核异常及碱性磷酸酶和超氧化物歧化酶活性的影响.海洋湖沼通报,000(4),45-51.
MLA	吴志昊,et al."水中亚铁对大菱鲆幼鱼红细胞核异常及碱性磷酸酶和超氧化物歧化酶活性的影响".海洋湖沼通报 000.4(2011):45-51.

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