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| Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination | |
| Wang, Yujuan1; Wang, Xiuhua1,2; Huang, Jie1,2; Li, Jun2,3,4 | |
| 2016-03-01 | |
| 发表期刊 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 卷号 | 17期号:3 |
| 文章类型 | Article |
| 摘要 | The adjuvant effect of Quillaja saponaria saponin (QSS) on protection of turbot fry was investigated with immersion vaccination of formalin-killed Vibrio anguillarum O1 and various concentrations of QSS (5, 25, 45 and 65 mg/L). Fish were challenged at days 7, 14 and 28 post-vaccination. Significantly high relative percent of survival (RPS) ((59.1 +/- 13.6)%, (81.7 +/- 8.2)%, (77.8 +/- 9.6)%) were recorded in the fish that received bacterins immersion with QSS at 45 mg/L, which is comparable to the positive control group vaccinated by intraperitoneal injection (IP). Moreover, a remarkably higher serum antibody titer was also demonstrated after 28 days in the vaccinated fish with QSS (45 mg/L) than those vaccinated fish without QSS (p < 0.05), but lower than the IP immunized fish (p < 0.05). Significant upregulation of IgM gene expression has also been identified in the tissues of skin, gill, spleen and kidney from the immunized fish in comparison to the control fish. Taken together, the present study indicated that QSS was able to dramatically evoke systemic and mucosal immune responses in immunized fish. Therefore, QSS might be a promising adjuvant candidate for fish vaccination via an immersion administering route. |
| 关键词 | Vaccination Relative Percent Of Survival (Rps) Quillaja Saponaria Saponins Scophthalmus Maximus Adjuvant |
| DOI | 10.3390/ijms17030325 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000373712800078 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/126271 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 4.Lake Super State Univ, Sch Biol Sci, Sault Ste Marie, MI 49783 USA |
| 推荐引用方式 GB/T 7714 | Wang, Yujuan,Wang, Xiuhua,Huang, Jie,et al. Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2016,17(3). |
| APA | Wang, Yujuan,Wang, Xiuhua,Huang, Jie,&Li, Jun.(2016).Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,17(3). |
| MLA | Wang, Yujuan,et al."Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 17.3(2016). |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Adjuvant Effect of Q（1210KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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