Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Two novel LRR-only proteins in Chlamys farreri: Similar in structure, yet different in expression profile and pattern recognition | |
Wang, Mengqiang1 ; Wang, Lingling1; Xin, Lusheng1,3; Wang, Xiudan1,3; Wang, Lin1,3; Xu, Jianchao1,3; Jia, Zhihao1,3; Yue, Feng1; Wang, Hao1; Song, Linsheng2
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| 2016-06-01 | |
| 发表期刊 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
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| 卷号 | 59页码:99-109 |
| 文章类型 | Article |
| 摘要 | Leucine-rich repeat (LRR)-only proteins could mediate protein-ligand and protein protein interactions and be involved in the immune response. In the present study, two novel LRR-only proteins, CfLRRop-2 and CfLRRop-3, were identified and characterized from scallop Chlamys farreri. They both contained nine LRR motifs with the consensus signature sequence LxxLxLxxNxL and formed typical horseshoe structure. The CfLRRop-2 and CfLRRop-3 mRNA transcripts were constitutively expressed in haemocytes, muscle, mantle, gill, haepatopancreas and gonad, with the highest expression level in haepatopancreas and gill, respectively. During the ontogenesis of scallop, the mRNA transcripts of CfLRRop-2 were kept at a high level in oocytes and embryos, while those of CfLRRop-3 were expressed at a rather low level from oocytes to blastula. Their mRNA transcripts were significantly increased after the stimulation of lipopolysaccharide (LPS), peptidoglycan (PGN), glucan (GLU) and polyinosinic-polycytidylic acid (poly I:C), and the mRNA expression of CfLRRop-2 rose more intensely than that of CfLRRop-3. After the suppression of CfTLR (previously identified Toll-like receptor in C. farreri) via RNA interference (RNAi), CfLRRop-3 mRNA transcripts increased more intensely and lastingly than those of CfLRRop-2. The rCfLRRop-3 protein could bind LPS, PGN, GLU and poly I:C, while rCfLRRop-2 exhibited no significant binding activity to them. Additionally, rCfLRRop-2 could significantly induce the release of TNF-alpha from the mixed primary cultured scallop haemocytes, but rCfLRRop-3 failed. These results collectively indicated that CfLRRop-2 might act as an immune effector or pro-inflammatory factor, while CfLRRop-3 would function as a pattern recognition receptor (PRR), suggesting the function of LRR-only protein family has differentiated in scallop. (C) 2016 Elsevier Ltd. All rights reserved. |
| 关键词 | Chlamys Farreri Innate Immunity Leucine-rich Repeat Lrr-only Protein Recognition Pattern |
| DOI | 10.1016/j.dci.2016.01.013 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000373547000011 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/126257 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Mengqiang,Wang, Lingling,Xin, Lusheng,et al. Two novel LRR-only proteins in Chlamys farreri: Similar in structure, yet different in expression profile and pattern recognition[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,59:99-109. |
| APA | Wang, Mengqiang.,Wang, Lingling.,Xin, Lusheng.,Wang, Xiudan.,Wang, Lin.,...&Song, Linsheng.(2016).Two novel LRR-only proteins in Chlamys farreri: Similar in structure, yet different in expression profile and pattern recognition.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,59,99-109. |
| MLA | Wang, Mengqiang,et al."Two novel LRR-only proteins in Chlamys farreri: Similar in structure, yet different in expression profile and pattern recognition".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 59(2016):99-109. |
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| Two novel LRR-only p（3273KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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