Structural properties of the tubular appendage spinae from marine bacterium Roseobacter sp strain YSCB

doi:10.1038/srep00950

	Structural properties of the tubular appendage spinae from marine bacterium Roseobacter sp strain YSCB
	Bernadac, A.2; Wu, L. -F.1,8; Santini, C. -L.1,8; Vidaud, C.3; Sturgis, J. N.4; Menguy, N.5,8; Bergam, P.2; Nicoletti, C.6; Xiao, T.7,8; Wu, LF (reprint author), Aix Marseille Univ, Inst Microbiol Mediterranee, CNRS, Lab Chim Bacterienne,UMR 7283, 31 Chem Joseph Aiguier, F-13402 Marseille 20, France.
	2012-12-10
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	2 页码:950
文章类型	Article
摘要	Spinae are tubular surface appendages broadly found in Gram-negative bacteria. Little is known about their architecture, function or origin. Here, we report structural characterization of the spinae from marine bacteria Roseobacter sp. YSCB. Electron cryo-tomography revealed that a single filament winds into a hollow flared base with progressive change to a cylinder. Proteinase K unwound the spinae into proteolysis-resistant filaments. Thermal treatment ripped the spinae into ribbons that were melted with prolonged heating. Circular dichroism spectroscopy revealed a dominant beta-structure of the spinae. Differential scanning calorimetry analyses showed three endothermic transformations at 50-85 degrees C, 98 degrees C and 123 degrees C, respectively. The heating almost completely disintegrated the spinae, abolished the 98 degrees C transition and destroyed the beta-structure. Infrared spectroscopy identified the amide I spectrum maximum at a position similar to that of amyloid fibrils. Therefore, the spinae distinguish from other bacterial appendages, e.g. flagella and stalks, in both the structure and mechanism of assembly.
关键词	Differential Scanning Calorimetry Amyloid Fibrils Protein Chlorobium Colonization Biogenesis Stability Larvae Clade
学科领域	Multidisciplinary Sciences
DOI	10.1038/srep00950
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000312273400009
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12442
专题	海洋生态与环境科学重点实验室
通讯作者	Wu, LF (reprint author), Aix Marseille Univ, Inst Microbiol Mediterranee, CNRS, Lab Chim Bacterienne,UMR 7283, 31 Chem Joseph Aiguier, F-13402 Marseille 20, France.
作者单位	1.Aix Marseille Univ, Inst Microbiol Mediterranee, CNRS, Lab Chim Bacterienne,UMR 7283, F-13402 Marseille 20, France 2.Aix Marseille Univ, Inst Microbiol Mediterranee, CNRS, Serv Microscopie Elect, F-13402 Marseille 20, France 3.CEA Marcoule, Inst Biol Environm & Biotechnol, Lab Target Prot Studies, F-30207 Bagnolssur Ceze, France 4.Aix Marseille Univ, Inst Microbiol Mediterranee, CNRS, Lab Ingn Syst Macromol,UMR 7255, F-13402 Marseille 20, France 5.Univ Paris 06, IMPMC, UMR 7590, CNRS,IPGP,IRD, Paris 05, France 6.Aix Marseille Univ, CNRS, ISM2, UMR 7313, F-13397 Marseille 20, France 7.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 8.CNRS, Lab Int Assoc Biomineralisat & Nanostruct LIA Bio, F-13402 Marseille 20, France
推荐引用方式 GB/T 7714	Bernadac, A.,Wu, L. -F.,Santini, C. -L.,et al. Structural properties of the tubular appendage spinae from marine bacterium Roseobacter sp strain YSCB[J]. SCIENTIFIC REPORTS,2012,2:950.
APA	Bernadac, A..,Wu, L. -F..,Santini, C. -L..,Vidaud, C..,Sturgis, J. N..,...&Wu, LF .(2012).Structural properties of the tubular appendage spinae from marine bacterium Roseobacter sp strain YSCB.SCIENTIFIC REPORTS,2,950.
MLA	Bernadac, A.,et al."Structural properties of the tubular appendage spinae from marine bacterium Roseobacter sp strain YSCB".SCIENTIFIC REPORTS 2(2012):950.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Structural propertie（4610KB）			限制开放	使用许可	浏览