Institutional Repository of Key Laboratory of Marine Ecology & Environmental Sciences, CAS
| Gel microbead cultivation with a subenrichment procedure can yield better bacterial cultivability from a seawater sample than standard plating method | |
| Ji Shiqi1; Zhao Rui1; Yin Qi1; Zhao Yuan2; Liu Chenguang1; Xiao Tian2; Zhang Xiaohua1; Zhang, XH (reprint author), Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China. | |
| 2012-03-01 | |
| 发表期刊 | JOURNAL OF OCEAN UNIVERSITY OF CHINA
 |
| ISSN | 1672-5182 |
| 卷号 | 11期号:1页码:45-51 |
| 文章类型 | Article |
| 摘要 | A gel microbead (GMD) cultivation method was employed to cultivate microorganisms from an amphioxus breeding zone in Qingdao, P. R. China. The culture results were compared with those by standard plating method. In the GMD-based method, the microcolony-forming GMDs were sorted by fluorescence-activated cell sorting (FACS). To further get pure cultures, a subsequent enrichment culture and a streaking purification procedure were conducted on marine R2A medium. Eighty bacterial strains isolated by the GMD-based method were randomly selected for sequencing. These isolates belonged to Alphaproteobacteria (33%), Gammaproteobacteria (44%), Bacteroidetes (11%), Actinobacteria (5%), Firmicutes (5%), Epsilonproteobacteria (1%), and Verrucomicrobia (1%), the last two groups being usually difficult to culture. The 16S rRNA gene sequences revealed a diverse community with 91.1%-100% of the bacterial rRNAs similarities. Thirteen strains were sharing 16S rRNA gene sequence which was less than 97% similar to any other rRNA genes currently deposited in TYP16S database. Seventy isolates derived from the standard plating method fell into 4 different taxonomic groups: Alphaproteobacteria (9%), Gammaproteobacteria (81%), Bacteroidetes (7%) and Firmicutes (3%) with a 16S rRNA gene sequence similarities between 95.8%-100%, in which only 3 strains were sharing 16S rRNA gene sequence of less than 97%. The results indicated that the GMD-based method with subenrichment culture yielded more taxonomic groups and more novel microbial strains, including members of previously rarely cultured groups, when compared with the standard plating method, and that this method markedly improved the bacterial cultivability. |
| 关键词 | Gmd Cultivation Marine Microorganism Bacterial Cultivability Amphioxus |
| 学科领域 | Oceanography |
| DOI | 10.1007/s11802-012-1869-y |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000299527100007 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/12430 |
| 专题 | 海洋生态与环境科学重点实验室 |
| 通讯作者 | Zhang, XH (reprint author), Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China. |
| 作者单位 | 1.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ji Shiqi,Zhao Rui,Yin Qi,et al. Gel microbead cultivation with a subenrichment procedure can yield better bacterial cultivability from a seawater sample than standard plating method[J]. JOURNAL OF OCEAN UNIVERSITY OF CHINA,2012,11(1):45-51. |
| APA | Ji Shiqi.,Zhao Rui.,Yin Qi.,Zhao Yuan.,Liu Chenguang.,...&Zhang, XH .(2012).Gel microbead cultivation with a subenrichment procedure can yield better bacterial cultivability from a seawater sample than standard plating method.JOURNAL OF OCEAN UNIVERSITY OF CHINA,11(1),45-51. |
| MLA | Ji Shiqi,et al."Gel microbead cultivation with a subenrichment procedure can yield better bacterial cultivability from a seawater sample than standard plating method".JOURNAL OF OCEAN UNIVERSITY OF CHINA 11.1(2012):45-51. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Gel microbead cultiv（444KB） | 限制开放 | 使用许可 | 浏览 | |||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论