Analysis of Purified Okadaic Acid and Dinophysis Toxin 1 from Prorocentrum Lima Dodge

doi:10.3724/SP.J.1096.2012.00741

	Analysis of Purified Okadaic Acid and Dinophysis Toxin 1 from Prorocentrum Lima Dodge
	Feng Zhen-Zhou 1,2; Yu Ren-Cheng1 ; Zhou Ming-Jiang 1; Yu, RC (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China.
	2012-04-01
发表期刊	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN	0253-3820
卷号	40 期号:4 页码:539-544
文章类型	Article
摘要	Okadaic acid (OA) and dinophysis toxins (DTXs) are representative toxins corresponding for the diarrhetic shellfish poisoning (DSP) incidents. In the current study, the samples of OA and DTX1, which were purified previously from the cultured algal cells of dinoflagellate Prorocentrum lima Dodge, were analyzed for the identity and purity with high performance liquid chromatography coupled with fluorescence detector (HPLC-FLD) and ultraviolet detector (HPLC-UV), mass spectrometry (MS) and HPLC-MS. Commercially available toxin standards of OA and DTX1 were also analyzed to compare with the purified samples. After derivatized with 4-bromomethyl-7-methoxycoumarin (BrMmc), OA and DTX1 were detected in the purified samples with the HPLC-FLD method. In the HPLC-UV chromatogrphy detected at 200 nm, OA and DTX1 were found to be the most predominant peaks, and the purity calculated based on the peak area could reach 95% and 98% for the samples of OA and DTX1, respectively. In the MS spectrums of the purified samples and toxin standards, the highest signals in the positive mode or the negative mode had the same value of m/z, which could be identified as [M+Na](+) and [M-H](-) of OA and DTX1. The [M+Na](+) and [M-H](-) ions of the toxin samples showed similar fragmentation pattern as those of the toxin standards, further confirming the presence of OA and DTX1 in the purified samples. In the LC-MS analysis of the purified samples with the full-scan m/z range at 100-1300, a small amount of impurities in the OA sample was found, and no impurity presented in the DTX1 sample. The results suggested that the OA and DTX1 samples prepared previously had high purity and could be used as standards for the analysis of OA and DTX1 with HPLC-FLD and HPLC-MS methods in management and protection of seafood.
关键词	Diarrhetic Shellfish Poisoning Okadaic Acid Dinophysis Toxin 1 High Performance Liquid Chromatography-mass Spectrometry
学科领域	Chemistry, Analytical
DOI	10.3724/SP.J.1096.2012.00741
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000313810800007
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12413
专题	海洋生态与环境科学重点实验室
通讯作者	Yu, RC (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Feng Zhen-Zhou,Yu Ren-Cheng,Zhou Ming-Jiang,et al. Analysis of Purified Okadaic Acid and Dinophysis Toxin 1 from Prorocentrum Lima Dodge[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2012,40(4):539-544.
APA	Feng Zhen-Zhou,Yu Ren-Cheng,Zhou Ming-Jiang,&Yu, RC .(2012).Analysis of Purified Okadaic Acid and Dinophysis Toxin 1 from Prorocentrum Lima Dodge.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,40(4),539-544.
MLA	Feng Zhen-Zhou,et al."Analysis of Purified Okadaic Acid and Dinophysis Toxin 1 from Prorocentrum Lima Dodge".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 40.4(2012):539-544.

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