The Hypoglycemic Effect of the Kelp on Diabetes Mellitus Model Induced by Alloxan in Rats

doi:10.3390/ijms13033354

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	The Hypoglycemic Effect of the Kelp on Diabetes Mellitus Model Induced by Alloxan in Rats
	Long, Shao-Hua 1; Yu, Zhu-Qin 1; Shuai, Li 2; Guo, Yun-Liang 1; Duan, De-Lin 3; Xu, Xin-Ying 1; Li, Xiao-Dan 1; Guo, YL (reprint author), Qingdao Univ, Coll Med, Affiliated Hosp, Inst Cerebrovasc Dis, Qingdao 266003, Shandong, Peoples R China.
	2012-03-01
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN	1661-6596
卷号	13 期号:3 页码:3354-3365
文章类型	Article
摘要	Hypoglycemic effects and the use of kelp in diabetes mellitus (DM) model rats induced by alloxan were investigated. Sixty healthy male rats were used to establish DM models by injecting alloxan intraperitoneally. Kelp powder was added to the general forage for the rats. The levels of fasting blood glucose (FBG) were determined by an automatic blood glucose device. Electrochemiluminescence immunoassay was applied to determine the serum levels of insulin. The serum levels of malondialdehyde (MDA) were measured by thiobarbituric acid assay and nitric oxide (NO) by nitrate reductase assay. The activities of superoxide dismutase (SOD) were determined by xanthinoxidase assay and glutathione peroxidase (GSH-Px) by chemical colorimetry. The shape and structure of islet cells were observed with Hematine-Eosin staining, and the expression of superoxide dismutase (SOD) and inducible nitric oxide synthase (iNOS) in islet cells were detected by immunohistochemical assay. The results showed that the serum levels of insulin after treatment with kelp powder increased significantly compared to those in the DM-model group, while the FBG in the medium-high dose treated groups decreased significantly compared to those in the DM-model group (P < 0.05). The levels of MDA and NO in the kelp powder groups were lower than those in the DM-model group, while the activities of SOD and GSH-Px were higher than those in the DM-model group, of which a significant difference existed between the medium-high dose treated groups and the DM-model group (P < 0.05). The shape and structure of islet cells improved with the up-expressing SOD and down-expressing iNOS in the medium-high dose treated groups compared to those in the DM-model group (P < 0.05). There were no significant differences between the medium and high dose treated groups, all above indexes (P > 0.05). It is suggested that kelp might aid recovery of the the islet cell secreting function and reduce the level of FBG by an antioxidant effect.
关键词	Kelp Diabetes Mellitus Alloxan Oxidative Stress Rats
学科领域	Chemistry, Multidisciplinary
DOI	10.3390/ijms13033354
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000302174500047
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12395
专题	实验海洋生物学重点实验室
通讯作者	Guo, YL (reprint author), Qingdao Univ, Coll Med, Affiliated Hosp, Inst Cerebrovasc Dis, Qingdao 266003, Shandong, Peoples R China.
作者单位	1.Qingdao Univ, Coll Med, Affiliated Hosp, Inst Cerebrovasc Dis, Qingdao 266003, Shandong, Peoples R China 2.Qingdao Univ, Sch Chem Chem Engn & Environm Sci, Qingdao 266071, Shandong, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Long, Shao-Hua,Yu, Zhu-Qin,Shuai, Li,et al. The Hypoglycemic Effect of the Kelp on Diabetes Mellitus Model Induced by Alloxan in Rats[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2012,13(3):3354-3365.
APA	Long, Shao-Hua.,Yu, Zhu-Qin.,Shuai, Li.,Guo, Yun-Liang.,Duan, De-Lin.,...&Guo, YL .(2012).The Hypoglycemic Effect of the Kelp on Diabetes Mellitus Model Induced by Alloxan in Rats.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,13(3),3354-3365.
MLA	Long, Shao-Hua,et al."The Hypoglycemic Effect of the Kelp on Diabetes Mellitus Model Induced by Alloxan in Rats".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 13.3(2012):3354-3365.

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