Susceptibility of a Vibrio alginolyticus rpoS mutant to environmental stresses and its expression of OMPs

doi:10.1002/jobm.201100249

IOCAS-IR > 实验海洋生物学重点实验室

	Susceptibility of a Vibrio alginolyticus rpoS mutant to environmental stresses and its expression of OMPs
	Wang Shuxian 1; Wei Jianteng 2; Li Tianbao 1; Wang, SX (reprint author), Mariculture Inst Shandong Prov, Shandong Prov Key Lab Dis Control Mariculture, 47 Guizhou Rd, Qingdao, Shandong, Peoples R China.
	2012-08-01
发表期刊	JOURNAL OF BASIC MICROBIOLOGY
ISSN	0233-111X
卷号	52 期号:4 页码:467-476
文章类型	Article
摘要	Vibrio alginolyticus, one of the most important opportunistic pathogens, can be detected in human being and marine animals. Like other bacteria, V. alginolyticus is able to adapt to a variety of stressful environmental changes. The alternate sigma factor RpoS, which is a regulator during stationary phase, plays an important role in surviving under these stressful situations in many bacteria. Sequence analysis reveals a 990 bp open reading frame which is predicted to encode a 330-amino-acid protein with 68% to 96% overall identity to other reported sequences. To study the function of rpoS, the rpoS gene of V. alginolyticus VIB283 was cloned and an rpoS mutant was constructed by homologous recombination. Comparison of the study result of the wild type and the mutant showed that the mutant was more sensitive to stress conditions such as high osmolarity, oxidative stress, heat shock, and long-term starvation and that the LD50 of the mutant strain to the zebra fish was about 2.8 times as that of the control strain. In addition, the SDS-PAGE analysis indicated that the outer membrane proteins (OMPs) existed great differences. ((c) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
关键词	Rpos Susceptibility Environmental Stresses Outer Membrane Proteins Vibrio Alginolyticus
学科领域	Microbiology
DOI	10.1002/jobm.201100249
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000307162200012
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12389
专题	实验海洋生物学重点实验室
通讯作者	Wang, SX (reprint author), Mariculture Inst Shandong Prov, Shandong Prov Key Lab Dis Control Mariculture, 47 Guizhou Rd, Qingdao, Shandong, Peoples R China.
作者单位	1.Mariculture Inst Shandong Prov, Shandong Prov Key Lab Dis Control Mariculture, Qingdao, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Wang Shuxian,Wei Jianteng,Li Tianbao,et al. Susceptibility of a Vibrio alginolyticus rpoS mutant to environmental stresses and its expression of OMPs[J]. JOURNAL OF BASIC MICROBIOLOGY,2012,52(4):467-476.
APA	Wang Shuxian,Wei Jianteng,Li Tianbao,&Wang, SX .(2012).Susceptibility of a Vibrio alginolyticus rpoS mutant to environmental stresses and its expression of OMPs.JOURNAL OF BASIC MICROBIOLOGY,52(4),467-476.
MLA	Wang Shuxian,et al."Susceptibility of a Vibrio alginolyticus rpoS mutant to environmental stresses and its expression of OMPs".JOURNAL OF BASIC MICROBIOLOGY 52.4(2012):467-476.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Susceptibility of a （573KB）			限制开放	使用许可	浏览