siRNA targeting Notch-1 decreases glioma stem cell proliferation and tumor growth

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	siRNA targeting Notch-1 decreases glioma stem cell proliferation and tumor growth
	Wang, Jianpeng; Wang, Chao; Meng, Qinghai; Li, Shifang; Sun, Xiaopeng; Bo, Yongli; Yao, Weicheng; Yao, WC (reprint author), Qingdao Univ, Coll Med, Affiliated Hosp, Dept Neurosurg, 16 Jiangsu Rd, Qingdao 266003, Shandong, Peoples R China.
	2012
发表期刊	MOLECULAR BIOLOGY REPORTS
ISSN	0301-4851
卷号	39 期号:3 页码:2497-2503
摘要	Glioblastoma multiforme (GBM), the most common brain tumor in adults, is neurologically destructive and has a dismal response to virtually all therapeutic modalities. One phenomenon that can contribute to this complexity is the presence of a relatively small subset of glioma stem cells (GSCs) within the tumor and the activation of pathways that control cellular differentiation. The Notch signaling pathway, which is responsible for maintaining a balance between cell proliferation and apoptosis, is believed to be deregulated in cancer stem cells (CSCs), leading to tumor growth through the generation or expansion of CSCs. In this study, Notch-1 small interfering RNA (siRNA) was used to silence Notch-1 gene expression in GSCs. An MTT assay demonstrated inhibitory effects on the proliferation of GSCs in vitro. Real-time PCR showed that Notch-1 expression levels were markedly decreased in GSCs transfected with Notch-1 siRNA in vitro. Notch-1 silenced GSCs engrafted on Balb/c nude mice showed a significantly greater reduction in oncogenicity than the control group (P < 0.05). Furthermore, direct intratumoral injections of Notch-1-siRNA/PEI significantly delayed the growth of pre-established tumors in nude mice (P < 0.05). These results suggest that siRNA-mediated silencing of the Notch-1 gene may represent a novel target for gene therapy of GBM.
关键词	Initiating Cells Glioblastoma-multiforme Side Population Breast-cancer Pathway Expression Xenografts Activation Phenotype Survival
学科领域	Biochemistry & Molecular Biology
URL	查看原文
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12385
专题	实验海洋生物学重点实验室
通讯作者	Yao, WC (reprint author), Qingdao Univ, Coll Med, Affiliated Hosp, Dept Neurosurg, 16 Jiangsu Rd, Qingdao 266003, Shandong, Peoples R China.
推荐引用方式 GB/T 7714	Wang, Jianpeng,Wang, Chao,Meng, Qinghai,et al. siRNA targeting Notch-1 decreases glioma stem cell proliferation and tumor growth[J]. MOLECULAR BIOLOGY REPORTS,2012,39(3):2497-2503.
APA	Wang, Jianpeng.,Wang, Chao.,Meng, Qinghai.,Li, Shifang.,Sun, Xiaopeng.,...&Yao, WC .(2012).siRNA targeting Notch-1 decreases glioma stem cell proliferation and tumor growth.MOLECULAR BIOLOGY REPORTS,39(3),2497-2503.
MLA	Wang, Jianpeng,et al."siRNA targeting Notch-1 decreases glioma stem cell proliferation and tumor growth".MOLECULAR BIOLOGY REPORTS 39.3(2012):2497-2503.

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