Overexpression of NF-kappa B inhibitor alpha in Cynoglossus semilaevis impairs pathogen-induced immune response

doi:10.1016/j.dci.2011.08.001

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	Overexpression of NF-kappa B inhibitor alpha in Cynoglossus semilaevis impairs pathogen-induced immune response
	Zhang, Min; Xiao, Zhi-zhong; Sun, Li; Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
	2012
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	36 期号:1 页码:253-257
文章类型	Article
摘要	I kappa B alpha is a member of the NF-kappa B inhibitor family that inhibits NF-kappa B activity by sequestering NF-kappa B in an inactive form in the cytosol. Unlike mammalian I kappa B alpha, which has been extensively studied, very little is known about the function of fish I kappa B alpha. In this study, we identified and analyzed an I kappa B alpha homologue, CsI kappa B alpha from half-smooth tongue sole (Cynoglossus semilaevis), a marine flatfish with important economic value. The deduced amino acid sequence of CsI kappa B alpha contains 308 residues and shares 58-82% overall sequence identities with the I kappa B alpha of a number of teleosts. In silico analysis identified in CsI kappa B alpha conserved domains that in mammals are known to be involved in phosphorylation, ubiquitination, and degradation of I kappa B alpha. Quantitative real time RT-PCR detected constitutive expression of CsI kappa B alpha in gut, spleen, liver, gill, heart, brain, muscle, and kidney. Experimental challenge with a bacterial pathogen-induced significant inductions of CsI kappa B alpha expression in head and trunk kidney, which, however, were transient and much lower in magnitude than that of interleukin-1 beta. To examine the effect of unregulated overexpression of Csl kappa B alpha in a live fish model, tongue sole were administered via intramuscular injection with plasmid pCNCsIkBa, which constitutively expresses CsI kappa B alpha. PCR, RT-PCR, and immunohistochemistry analysis showed that pCNCsIkBa was able to translocate to internal tissues, where transcription and translation of the recombinant CsI kappa B alpha took place. Compared to control fish, fish administered with pCNCsIkBa were impaired in the ability to block bacterial dissemination and survival in kidney and exhibited significantly reduced expression of multiple immune genes. These results suggest the possible existence in tongue sole of a NF-kappa B-I kappa B alpha signaling pathway that is negatively regulated by CsI kappa B alpha and required for effective defense against bacterial infection. (C) 2011 Elsevier Ltd. All rights reserved.
关键词	Nf-kappa b Inhibitor Cynoglossus Semilaevis Immune Response
学科领域	Immunology ; Zoology
DOI	10.1016/j.dci.2011.08.001
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000299390300030
引用统计	被引频次：20[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12368
专题	实验海洋生物学重点实验室
通讯作者	Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
作者单位	Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Zhang, Min,Xiao, Zhi-zhong,Sun, Li,et al. Overexpression of NF-kappa B inhibitor alpha in Cynoglossus semilaevis impairs pathogen-induced immune response[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2012,36(1):253-257.
APA	Zhang, Min,Xiao, Zhi-zhong,Sun, Li,&Sun, L .(2012).Overexpression of NF-kappa B inhibitor alpha in Cynoglossus semilaevis impairs pathogen-induced immune response.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,36(1),253-257.
MLA	Zhang, Min,et al."Overexpression of NF-kappa B inhibitor alpha in Cynoglossus semilaevis impairs pathogen-induced immune response".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 36.1(2012):253-257.

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