Molecular characterization of Cynoglossus semilaevis CD28

doi:10.1016/j.fsi.2012.02.021

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	Molecular characterization of Cynoglossus semilaevis CD28
	Hu, Yong-hua; Sun, Bo-guang; Deng, Tian; Sun, Li; Sun, L (reprint author), Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
	2012-05-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	32 期号:5 页码:934-938
文章类型	Article
摘要	T lymphocyte activation requires a combination of signals, one of which is provided by interaction between CD28 and its ligands on antigen presenting cells. Although CD28-like sequences have been identified in a few teleosts, the function of fish CD28 is virtually unknown. In this study, we cloned and analyzed a CD28 gene, CsCD28, from half-smooth tongue sole (Cynoglossus semilaevis). The deduced amino acid sequence of CsCD28 contains 229 residues and shares 20.2%-40.3% overall sequence identities with known fish CD28 sequences. CsCD28 possesses structural features conserved in mammalian and teleost CD28, which include the MYPPPY motif in the extracellular immunoglobulin-like domain and the YXN motif in the cytoplasmic domain. The CsCD28 gene is 2746 bp and composed of four exons and three introns, which in organization resemble those of mammalian and trout CD28. Quantitative real time RT-PCR analysis showed that CsCD28 expression occurred predominately in kidney, spleen, gut, and gill. CsCD28 is localized on the surface of head kidney lymphocytes, and antibody ligation of CsCD28 induced significant levels of cellular proliferation. Taken together, these results indicate that CsCD28 is similar to mammalian CD28 in genetic and protein structures and possibly plays a role in T cell activation. (C) 2012 Elsevier Ltd. All rights reserved.
关键词	Cynoglossus Semilaevis Cd28 t Cell Activation
学科领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2012.02.021
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000302667200038
引用统计	被引频次：25[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12362
专题	实验海洋生物学重点实验室
通讯作者	Sun, L (reprint author), Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
作者单位	Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Hu, Yong-hua,Sun, Bo-guang,Deng, Tian,et al. Molecular characterization of Cynoglossus semilaevis CD28[J]. FISH & SHELLFISH IMMUNOLOGY,2012,32(5):934-938.
APA	Hu, Yong-hua,Sun, Bo-guang,Deng, Tian,Sun, Li,&Sun, L .(2012).Molecular characterization of Cynoglossus semilaevis CD28.FISH & SHELLFISH IMMUNOLOGY,32(5),934-938.
MLA	Hu, Yong-hua,et al."Molecular characterization of Cynoglossus semilaevis CD28".FISH & SHELLFISH IMMUNOLOGY 32.5(2012):934-938.

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