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| Induction of Apoptosis, G(0)/G(1) Phase Arrest and Microtubule Disassembly in K562 Leukemia Cells by Mere15, a Novel Polypeptide from Meretrix meretrix Linnaeus | |
| Liu, Ming1; Zhao, Xiangzhong2; Zhao, Jin1; Xiao, Lin1; Liu, Haizhou1; Wang, Cuicui1; Cheng, Linyou1; Wu, Ning1; Lin, Xiukun1; Lin, XK (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. | |
| 2012-11-01 | |
| 发表期刊 | MARINE DRUGS
 |
| ISSN | 1660-3397 |
| 卷号 | 10期号:11页码:2596-2607 |
| 文章类型 | Article |
| 摘要 | Mere15 is a novel polypeptide from Meretrix meretrix Linnaeus with cytotoxicity in solid cancer cells. In this study, we investigated its activity on human K562 chronic myelogenous leukemia cells. Mere15 inhibited the growth of K562 cells with IC50 values of 38.2 mu g/mL. Mere15 also caused concentration dependent induction of apoptosis, with overproduction of reactive oxygen species and loss of mitochondrial membrane potential. Moreover, Mere15 arrested cell cycle progression at G(0)/G(1) phase of K562 cells in a concentration dependent manner. In addition, Mere15 caused the disassembly of the microtubule cytoskeleton in K562 cells and inhibited the polymerization of tubulin in a cell free system via interaction with tubulin. We concluded that Mere15 was cytotoxic to K562 leukemia cells and the cytotoxicity was related to the apoptosis induction, cell cycle arrest and microtubule disassembly. These results implied that Merer15 was a broad spectrum anticancer polypeptide, not only cytotoxic to various solid cancer cells but also to the chronic myelogenous leukemia cells. Mere15 may have therapeutic potential for the treatment of leukemia. |
| 关键词 | Meretrix Meretrix Linnaeus Mere15 Apoptosis Anticancer |
| 学科领域 | Chemistry, Medicinal |
| DOI | 10.3390/md10112596 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000311425800016 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/12353 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Lin, XK (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Shandong Acad Med Sci, Key Lab Rare Dis Shandong Prov, Inst Basic Med, Jinan 250062, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Liu, Ming,Zhao, Xiangzhong,Zhao, Jin,et al. Induction of Apoptosis, G(0)/G(1) Phase Arrest and Microtubule Disassembly in K562 Leukemia Cells by Mere15, a Novel Polypeptide from Meretrix meretrix Linnaeus[J]. MARINE DRUGS,2012,10(11):2596-2607. |
| APA | Liu, Ming.,Zhao, Xiangzhong.,Zhao, Jin.,Xiao, Lin.,Liu, Haizhou.,...&Lin, XK .(2012).Induction of Apoptosis, G(0)/G(1) Phase Arrest and Microtubule Disassembly in K562 Leukemia Cells by Mere15, a Novel Polypeptide from Meretrix meretrix Linnaeus.MARINE DRUGS,10(11),2596-2607. |
| MLA | Liu, Ming,et al."Induction of Apoptosis, G(0)/G(1) Phase Arrest and Microtubule Disassembly in K562 Leukemia Cells by Mere15, a Novel Polypeptide from Meretrix meretrix Linnaeus".MARINE DRUGS 10.11(2012):2596-2607. |
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