Edwardsiella tarda sialidase: Pathogenicity involvement and vaccine potential

doi:10.1016/j.fsi.2012.06.002

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	Edwardsiella tarda sialidase: Pathogenicity involvement and vaccine potential
	Jin, Ren-ping 1,2; Hu, Yong-hua 1; Sun, Bo-guang1 ; Zhang, Xiao-hua 2; Sun, Li 1; Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
	2012-09-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	33 期号:3 页码:514-521
文章类型	Article
摘要	Bacterial sialidases are a group of glycohydrolases that are known to play an important role in invasion of host cells and tissues. In this study, we examined in a model of Japanese flounder (Paralichthys olivaceus) the potential function of NanA, a sialidase from the fish pathogen Edwardsiella tarda. NanA is composed of 670 residues and shares low sequence identities with known bacterial sialidases. In silico analysis indicated that NanA possesses a sialidase domain and an autotransporter domain, the former containing five Asp-boxes, a RIP motif, and the conserved catalytic site of bacterial sialidases. Purified recombinant NanA (rNanA) corresponding to the sialidase domain exhibited glycohydrolase activity against sialic acid substrate in a manner that is pH and temperature dependent. Immunofluorescence microscopy showed binding of anti-rNanA antibodies to E. tarda, suggesting that NanA was localized on cell surface. Mutation of nanA caused drastic attenuation in the ability of E. tarda to disseminate into and colonize fish tissues and to induce mortality in infected fish. Likewise, cellular study showed that the nanA mutant was significantly impaired in the infectivity against cultured flounder cells. Immunoprotective analysis showed that rNanA in the form of a subunit vaccine conferred effective protection upon flounder against lethal E. tarda challenge. rNanA vaccination induced the production of specific serum antibodies, which enhanced complement-mediated bactericidal activity and reduced infection of E. tarda into flounder cells. Together these results indicate that NanA plays an important role in the pathogenesis of E tarda and may be exploited for the control of E. tarda infection in aquaculture. (C) 2012 Elsevier Ltd. All rights reserved.
关键词	Neuraminidase Edwardsiella Tarda Virulence Vaccine
学科领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2012.06.002
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000308057700006
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12316
专题	实验海洋生物学重点实验室
通讯作者	Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Ocean Univ China, Dept Marine Biol, Qingdao, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Jin, Ren-ping,Hu, Yong-hua,Sun, Bo-guang,et al. Edwardsiella tarda sialidase: Pathogenicity involvement and vaccine potential[J]. FISH & SHELLFISH IMMUNOLOGY,2012,33(3):514-521.
APA	Jin, Ren-ping,Hu, Yong-hua,Sun, Bo-guang,Zhang, Xiao-hua,Sun, Li,&Sun, L .(2012).Edwardsiella tarda sialidase: Pathogenicity involvement and vaccine potential.FISH & SHELLFISH IMMUNOLOGY,33(3),514-521.
MLA	Jin, Ren-ping,et al."Edwardsiella tarda sialidase: Pathogenicity involvement and vaccine potential".FISH & SHELLFISH IMMUNOLOGY 33.3(2012):514-521.

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