Crustins from eyestalk cDNA library of swimming crab Portunus trituberculatus: Molecular characterization, genomic organization and expression analysis

doi:10.1016/j.fsi.2012.08.002

IOCAS-IR > 海洋生物技术研发中心

	Crustins from eyestalk cDNA library of swimming crab Portunus trituberculatus: Molecular characterization, genomic organization and expression analysis
	Cui, Zhaoxia 1; Song, Chengwen1,2 ; Liu, Yuan 1; Wang, Shuangyan 1,2; Li, Qianqian1,2 ; Li, Xihong 1,2; Cui, ZX (reprint author), Chinese Acad Sci, Inst Oceanol, EMBL, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
	2012-10-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	33 期号:4 页码:937-945
文章类型	Article
摘要	Crustins are cationic, cysteine-rich antimicrobial proteins, containing a single whey acidic protein (WAP) domain in the C-terminal end. Different from the reported Ptcrustin in the hemocytes, two novel crustin genes (PtCrustin2 and PtCrustin3) were cloned completely from the eyestalk cDNA library of Portunus trituberculatus in this study. All PtCrustins share the consensus cysteine motif and are considered as Type I crustins. Four exons and three introns are identified in genomic DNA sequence of PtCrustin3 while three exons and two introns in PtCrustin2. The mRNA transcripts of PtCrustin2 and PtCrustin3 are mainly detected in eyestalk and gills, but not in hemocytes. Although both PtCrutins are up-regulated after challenge of three microorganisms, PtCrustin3 seems to respond more quickly to microbial challenge than Ptcrustin2. Unlike most crustins, both recombinant PtCrustin2 and PtCrustin3 exhibit antibacterial activity against Gram-positive bacteria Micrococcus luteus and Staphyloccocus aureus and Gram-negative bacterium Pseudomonas aeruginosa. In addition, rPtCrustin2 is moderately active against yeast Pichia pastoris and rPtCrustin3 show significant activity against Gram-negative bacterium Vibrio alginolyticus. These results indicate that PtCrustin2 and PtCrustin3 are two novel crustins and play different roles in immune response of P. trituberculatus against microbial challenge. (C) 2012 Elsevier Ltd. All rights reserved.
关键词	Portunus Trituberculatus Crustin Genomic Organization Expression Profile Antibacterial Activity
学科领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2012.08.002
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000310185800030
引用统计	被引频次：30[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12308
专题	海洋生物技术研发中心实验海洋生物学重点实验室
通讯作者	Cui, ZX (reprint author), Chinese Acad Sci, Inst Oceanol, EMBL, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, EMBL, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Cui, Zhaoxia,Song, Chengwen,Liu, Yuan,et al. Crustins from eyestalk cDNA library of swimming crab Portunus trituberculatus: Molecular characterization, genomic organization and expression analysis[J]. FISH & SHELLFISH IMMUNOLOGY,2012,33(4):937-945.
APA	Cui, Zhaoxia.,Song, Chengwen.,Liu, Yuan.,Wang, Shuangyan.,Li, Qianqian.,...&Cui, ZX .(2012).Crustins from eyestalk cDNA library of swimming crab Portunus trituberculatus: Molecular characterization, genomic organization and expression analysis.FISH & SHELLFISH IMMUNOLOGY,33(4),937-945.
MLA	Cui, Zhaoxia,et al."Crustins from eyestalk cDNA library of swimming crab Portunus trituberculatus: Molecular characterization, genomic organization and expression analysis".FISH & SHELLFISH IMMUNOLOGY 33.4(2012):937-945.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
1-s2.0-S105046481200（1858KB）			限制开放	CC BY-NC-SA	浏览