Combinational biosynthesis and characterization of a fluorescent 82 beta-phycocyanin of Spirulina platensis

doi:10.1007/s11434-012-5264-2

IOCAS-IR > 实验海洋生物学重点实验室

	Combinational biosynthesis and characterization of a fluorescent 82 beta-phycocyanin of Spirulina platensis
	Guan XiangYu 2; Zhang WeiJie 3; Chi XiaoYuan 3; Lin HanZhi 3,4; Wang JinFeng3 ; Qin Song 1; Qin, S (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China.
	2012-09-01
发表期刊	CHINESE SCIENCE BULLETIN
ISSN	1001-6538
卷号	57 期号:25 页码:3294-3299
文章类型	Article
摘要	To biosynthesize fluorescent Spirulina platensis (Sp) beta-phycocyanin (PC) in Escherichia coli, a BLASTP search for homologs of the cpeS gene, a chromophore lyase, was performed against the Synechocystis sp. PCC 6803 (S6) proteome. A highly homologous gene, slr2049, was obtained from the S6 genome. Sites 82 and 153 in beta-phycocyanin of Sp were modified by site-directed mutagenesis. Two recombinant expression vectors were constructed and transformed into E. coli BL21: (i) pCDF-cpcB (C153A)-slr2049-sll0583-ho1-pcyA; and (ii) pCDF-cpcB (C82I)-slr2049-sll0583-ho1-pcyA. Lyases encoded by the genes slr2049 and sll0583 catalyzed the linking of Sp 82 beta-PC to phycocyanobilin (PCB), and fluorescent CpcB (C153A)-PCB was generated. We present a strategy for the co-expression of multiple genes in a single expression vector to identify the function of an unknown gene. Recombinant phycobiliproteins produced on a large scale are promising fluorescent tags for diagnostics and pharmacology.
关键词	Escherichia Coli Phycocyanin Fluorescence Recombinant
学科领域	Multidisciplinary Sciences
DOI	10.1007/s11434-012-5264-2
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000307750500007
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12302
专题	实验海洋生物学重点实验室海洋生态与环境科学重点实验室
通讯作者	Qin, S (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China.
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.China Univ Geosci, Sch Ocean Sci, Beijing 100083, Peoples R China 3.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Guan XiangYu,Zhang WeiJie,Chi XiaoYuan,et al. Combinational biosynthesis and characterization of a fluorescent 82 beta-phycocyanin of Spirulina platensis[J]. CHINESE SCIENCE BULLETIN,2012,57(25):3294-3299.
APA	Guan XiangYu.,Zhang WeiJie.,Chi XiaoYuan.,Lin HanZhi.,Wang JinFeng.,...&Qin, S .(2012).Combinational biosynthesis and characterization of a fluorescent 82 beta-phycocyanin of Spirulina platensis.CHINESE SCIENCE BULLETIN,57(25),3294-3299.
MLA	Guan XiangYu,et al."Combinational biosynthesis and characterization of a fluorescent 82 beta-phycocyanin of Spirulina platensis".CHINESE SCIENCE BULLETIN 57.25(2012):3294-3299.

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