Cloning and transcriptional analysis of two sialic acid-binding lectins (SABLs) from razor clam Solen grandis

doi:10.1016/j.fsi.2012.01.012

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	Cloning and transcriptional analysis of two sialic acid-binding lectins (SABLs) from razor clam Solen grandis
	Yang, Jialong 1,3; Wei, Xiumei 2; Liu, Xiangquan 2; Xu, Jie 2; Yang, Dinglong 2; Yang, Jianmin 2; Fang, Jinghui 2; Hu, Xiaoke 1; Yang, JL (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China.
	2012-04-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	32 期号:4 页码:578-585
文章类型	Article
摘要	Sialic acid-binding lectin (SABL) plays crucial role in both innate and adaptive immune responses benefiting from its predominant affinity toward glycan. In the present study, two SABLs from razor clam Solen grandis (designated as SgSABL-1 and SgSABL-2) were identified, and their expression patterns, both in tissues and towards microorganism glycan stimulation, were then characterized. The cDNA of SgSABL-1 and SgSABL-2 was 988 and 1281 bp, containing an open reading frame (ORF) of 744 and 570 bp, respectively, and deduced amino acid sequences showed high similarity to other invertebrates SABLs. Both SgSABL-1 and SgSABL-2 encoded a C1q domain. SgSABL-1 and SgSABL-2 were found to be constitutively expressed in a wide range of tissues with different levels, including mantle, gill, gonad, hemocyte, muscle, and hepatopancreas, and both of them were highly expressed in hepatopancreas. SgSABL-1 and SgSABL-2 could be significantly induced after razor clams were stimulated by acetylated subunits-containing glycan LPS and PGN, suggesting the two SgSABLs might perform potential function of glycan recognition. In addition, SgSABL-2 could also be induced by beta-1,3-glucan. All these results indicated that SgSABL-1 and SgSABL-2 might be involved in the immune response against microbe infection and contributed to the pathogens recognition. (C) 2012 Elsevier Ltd. All rights reserved.
关键词	Solen Grandis Sialic Acid-binding Lectin Innate Immunity Sugar Recognition Real-time Pcr
学科领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2012.01.012
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000301827100009
引用统计	被引频次：21[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12301
专题	实验海洋生物学重点实验室
通讯作者	Yang, JL (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China.
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Shandong Marine Fisheries Res Inst, Yantai 264006, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Yang, Jialong,Wei, Xiumei,Liu, Xiangquan,et al. Cloning and transcriptional analysis of two sialic acid-binding lectins (SABLs) from razor clam Solen grandis[J]. FISH & SHELLFISH IMMUNOLOGY,2012,32(4):578-585.
APA	Yang, Jialong.,Wei, Xiumei.,Liu, Xiangquan.,Xu, Jie.,Yang, Dinglong.,...&Yang, JL .(2012).Cloning and transcriptional analysis of two sialic acid-binding lectins (SABLs) from razor clam Solen grandis.FISH & SHELLFISH IMMUNOLOGY,32(4),578-585.
MLA	Yang, Jialong,et al."Cloning and transcriptional analysis of two sialic acid-binding lectins (SABLs) from razor clam Solen grandis".FISH & SHELLFISH IMMUNOLOGY 32.4(2012):578-585.

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