Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Characterization and expression analysis of hsp70 gene from Ulva prolifera J. Agardh (Chlorophycophyta, Chlorophyceae) | |
Zhang, Haining1,2; Li, Wei1,2; Li, Jingjing1,2 ; Fu, Wandong1,3; Yao, Jianting1; Duan, Delin1; Duan, DL (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China.
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| 2012-03-01 | |
| 发表期刊 | MARINE GENOMICS
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| ISSN | 1874-7787 |
| 卷号 | 5页码:53-58 |
| 文章类型 | Article |
| 摘要 | In the Yellow Sea of China, large-scale green tides have broken out consecutively from 2007 to 2011. Ulva prohlera, the causative species of green tide, showed great ability to acclimate to adverse circumstance. To explore the mechanisms of rapid growth and stress resistance during the bloom, we characterized and analyzed hsp70 from U. prolifera. The results showed that hsp70 gene had 6 exons and 5 introns. The promoter-like region contained multiple cis-acting elements. The transcription of hsp70 was up-regulated by UV irradiation, heat treatment and salinities induction, but less influenced by desiccation. In vitro expression of HSP70 protein and western blot was also conducted, and the recombinant protein will be used in detecting the interaction between HSP70 and related functional proteins in the future. The study suggested that hsp70 could be used in prediction of stress tolerance in algae and monitoring environmental changes. (C) 2011 Elsevier B.V. All rights reserved. |
| 关键词 | Green Tide Ulva Prolifera Hsp70 Real-time Pcr In Vitro Expression |
| 学科领域 | Genetics & Heredity ; Marine & Freshwater Biology |
| DOI | 10.1016/j.margen.2011.10.001 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000301700900008 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/12294 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Duan, DL (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 3.Zhejiang Marine Dev Res Inst, Zhoushan, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, Haining,Li, Wei,Li, Jingjing,et al. Characterization and expression analysis of hsp70 gene from Ulva prolifera J. Agardh (Chlorophycophyta, Chlorophyceae)[J]. MARINE GENOMICS,2012,5:53-58. |
| APA | Zhang, Haining.,Li, Wei.,Li, Jingjing.,Fu, Wandong.,Yao, Jianting.,...&Duan, DL .(2012).Characterization and expression analysis of hsp70 gene from Ulva prolifera J. Agardh (Chlorophycophyta, Chlorophyceae).MARINE GENOMICS,5,53-58. |
| MLA | Zhang, Haining,et al."Characterization and expression analysis of hsp70 gene from Ulva prolifera J. Agardh (Chlorophycophyta, Chlorophyceae)".MARINE GENOMICS 5(2012):53-58. |
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