An iodothyronine deiodinase from Chlamys farreri and its induced mRNA expression after LPS stimulation

doi:10.1016/j.fsi.2012.05.011

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	An iodothyronine deiodinase from Chlamys farreri and its induced mRNA expression after LPS stimulation
	Wu, Tiantian 1,2; Shi, Xiaowei 1,2; Zhou, Zhi 1,2; Wang, Lingling 1; Wang, Mengqiang1,2 ; Wang, Leilei 1,2; Huang, Mengmeng1,2 ; Yang, Chuanyan 1,2; Song, Linsheng 1; Wang, LL (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
	2012-08-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	33 期号:2 页码:286-293
文章类型	Article
摘要	Iodothyronine deiodinase is responsible for the deiodination of thyroxine T4 to T3, and involved in the complex neuroendocrine-immune regulatory network to optimize the immune response in vertebrate. In this study, the full-length cDNA of an iodothyronine deiodinase (designated as CfDx) was cloned from scallop Chlamys farreri. The complete cDNA sequence of CfDx was of 1404 bp and contained an open reading frame of 900 bp encoding a polypeptide of 299 amino acids. The deduced amino acid sequence of CfDx contained an in-frame TGA stop codon probably encoding an essential selenocysteine (SeC), and there was a conserved region of about 15 amino acids surrounding the SeC residue. The CfDx mRNA transcripts were detected in all the tested tissues, including haemocytes, hepatopancreas, kidney, adductor muscle, gonad, gill and mantle, with the higher expression level in hepatopancreas and kidney. After LPS stimulation, the CfDx mRNA expression level in haemocytes increased significantly at 12 h (25.35-fold, P < 0.05) and 24 h (7.62-fold, P < 0.05), and the concentration of T3 in haemolymph increased significantly at 12 h (3.62 ng dL(-1), P < 0.05) even the concentration of T4 did not change significantly. After scallop received an injection of 50 mu g CfDx dsRNA, the expression level of CfDx mRNA in haemocytes began to decrease significantly at 36 h and maintained the relative low level (about 0.3-fold of the PBS control group) from 36 to 72 h, but the ratio of T4/T3 in haemolymph began to increase at 36 h (2.31-fold, P < 0.05) and kept increasing from 36 to 72 h comparing with that in the PBS control group. These results indicated that CfDx was a homologue of iodothyronine deiodinase in scallop C. Jarred, and it might be involved in the immunomodulation via regulating the concentration of thyroid hormones T3 and T4 in the haemolymph of scallop. (C) 2012 Elsevier Ltd. All rights reserved.
关键词	Chlamys Farreri Iodothyronine Deiodinase Thyroid Hormone Neuroendocrine Immunity
学科领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2012.05.011
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000306632800015
引用统计	被引频次：18[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12281
专题	实验海洋生物学重点实验室
通讯作者	Wang, LL (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Wu, Tiantian,Shi, Xiaowei,Zhou, Zhi,et al. An iodothyronine deiodinase from Chlamys farreri and its induced mRNA expression after LPS stimulation[J]. FISH & SHELLFISH IMMUNOLOGY,2012,33(2):286-293.
APA	Wu, Tiantian.,Shi, Xiaowei.,Zhou, Zhi.,Wang, Lingling.,Wang, Mengqiang.,...&Wang, LL .(2012).An iodothyronine deiodinase from Chlamys farreri and its induced mRNA expression after LPS stimulation.FISH & SHELLFISH IMMUNOLOGY,33(2),286-293.
MLA	Wu, Tiantian,et al."An iodothyronine deiodinase from Chlamys farreri and its induced mRNA expression after LPS stimulation".FISH & SHELLFISH IMMUNOLOGY 33.2(2012):286-293.

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