A trehalose-6-phosphate synthase gene from Chinese shrimp, Fenneropenaeus chinensis

doi:10.1007/s11033-012-1897-0

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	A trehalose-6-phosphate synthase gene from Chinese shrimp, Fenneropenaeus chinensis
	Zhang, Jiquan 1; Wang, Jing 1,2; Li, Fuhua 1; Sun, Yuying 1; Yang, Changjian 1; Xiang, Jianhai1 ; Zhang, JQ (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
	2012-12-01
发表期刊	MOLECULAR BIOLOGY REPORTS
ISSN	0301-4851
卷号	39 期号:12 页码:10219-10225
文章类型	Article
摘要	Trehalose is an important disaccharide and plays a key role in many organisms under different stress conditions. In the study, a gene (FcTPS) encoded trehalose-6-phosphate synthase was reported from Chinese shrimp, Fenneropenaeus chinensis. The full-length cDNA of FcTPS is 3,281 bp including a poly A-tail of 20 bp, encoding a putative protein of 844 amino acids. The predicted protein contains a glycol_transf_20 domain and a trehalose_PPase domain. Genomic structure of FcTPS is composed of three exons with 192, 157 and 2,912 bp and two introns with 1,057 and 568 bp. In the second intron, four different SSRs are found. Transcripts of FcTPS gene are constitutively expressed in various tissues, with strongest level in hepatopancreas. After the shrimp were challenged with WSSV or Vibrio and the expression of FcTPS in hepatopancreas were analyzed using real-time PCR, the result showed that FcTPS transcript was down-regulated significantly in response to the challenge of Vibro at the early of 5 h post-challenge and then up-regulated significantly at 14 h. In addition, the expression of FcTPS showed the same result after the shrimp were challenged with WSSV. These results provide some new information about the tissue distribution, expression profiles and potential function of the trehalose-6-phosphate synthase in shrimp.
关键词	Fenneropenaeus Chinensis Trehalose-6-phosphate Synthase Wssv Vibrio
学科领域	Biochemistry & Molecular Biology
DOI	10.1007/s11033-012-1897-0
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000310586700027
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12274
专题	实验海洋生物学重点实验室
通讯作者	Zhang, JQ (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhang, Jiquan,Wang, Jing,Li, Fuhua,et al. A trehalose-6-phosphate synthase gene from Chinese shrimp, Fenneropenaeus chinensis[J]. MOLECULAR BIOLOGY REPORTS,2012,39(12):10219-10225.
APA	Zhang, Jiquan.,Wang, Jing.,Li, Fuhua.,Sun, Yuying.,Yang, Changjian.,...&Zhang, JQ .(2012).A trehalose-6-phosphate synthase gene from Chinese shrimp, Fenneropenaeus chinensis.MOLECULAR BIOLOGY REPORTS,39(12),10219-10225.
MLA	Zhang, Jiquan,et al."A trehalose-6-phosphate synthase gene from Chinese shrimp, Fenneropenaeus chinensis".MOLECULAR BIOLOGY REPORTS 39.12(2012):10219-10225.

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