A TonB-dependent outer membrane receptor of Pseudomonas fluorescens: virulence and vaccine potential

doi:10.1007/s00203-012-0812-3

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	A TonB-dependent outer membrane receptor of Pseudomonas fluorescens: virulence and vaccine potential
	Hu, Yong-hua 1; Dang, Wei 1,2,3; Sun, Li 1; Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
	2012-09-01
发表期刊	ARCHIVES OF MICROBIOLOGY
ISSN	0302-8933
卷号	194 期号:9 页码:795-802
文章类型	Article
摘要	Pseudomonas fluorescens is a Gram-negative bacterium and a common aquaculture pathogen. In this study, we identified from a pathogenic P. fluorescens strain a TonB-dependent outer membrane receptor, TdrA, as a secreted protein and examined its function and vaccine potential. TdrA is composed of 746 residues and possesses conserved structural domains of TonB-dependent outer membrane receptors. Quantitative real-time reverse transcriptase-PCR analysis showed that expression of tdrA was upregulated under conditions of iron starvation and during infection of host cells. Consistently, iron depletion induced increased production of TdrA protein in the outer membrane. Compared to the wild type, a tdrA-knock out mutant (1) was unable to grow in the absence of iron, (2) exhibited drastically attenuated overall bacterial virulence, and (3) was impaired in the ability to establish lethal infection in host tissues. Purified recombinant TdrA (rTdrA), when used as a subunit vaccine to immunize flounder, was able to induce strong protective immunity, including production of serum-specific antibodies that resulted in effective protection against lethal-dose P. fluorescens challenge. Together, these results indicate that TdrA is an outer membrane receptor and a protective immunogen that is likely to be involved in iron acquisition and, as a result, required for optimal bacterial virulence.
关键词	Pseudomonas Fluorescens Tonb-dependent Receptor Virulence Vaccine
学科领域	Microbiology
DOI	10.1007/s00203-012-0812-3
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000307709100008
引用统计	被引频次：35[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12272
专题	实验海洋生物学重点实验室
通讯作者	Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Hangzhou Normal Univ, Coll Life & Environm Sci, Hangzhou Key Lab Anim Adaptat & Evolut, Hangzhou 310036, Zhejiang, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Hu, Yong-hua,Dang, Wei,Sun, Li,et al. A TonB-dependent outer membrane receptor of Pseudomonas fluorescens: virulence and vaccine potential[J]. ARCHIVES OF MICROBIOLOGY,2012,194(9):795-802.
APA	Hu, Yong-hua,Dang, Wei,Sun, Li,&Sun, L .(2012).A TonB-dependent outer membrane receptor of Pseudomonas fluorescens: virulence and vaccine potential.ARCHIVES OF MICROBIOLOGY,194(9),795-802.
MLA	Hu, Yong-hua,et al."A TonB-dependent outer membrane receptor of Pseudomonas fluorescens: virulence and vaccine potential".ARCHIVES OF MICROBIOLOGY 194.9(2012):795-802.

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