A multi-CRD C-type lectin with broad recognition spectrum and cellular adhesion from Argopecten irradians

doi:10.1016/j.dci.2011.10.002

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	A multi-CRD C-type lectin with broad recognition spectrum and cellular adhesion from Argopecten irradians
	Wang, Leilei 1,2; Wang, Lingling 1; Yang, Jialong 1,2; Zhang, Huan 1; Huang, Mengmeng1,2 ; Kong, Pengfei 1,2; Zhou, Zhi 1,2; Song, Linsheng 1; Wang, LL (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
	2012-03-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	36 期号:3 页码:591-601
文章类型	Article
摘要	C-type lectins are a superfamily of Ca2+-dependent carbohydrate-recognition proteins which play significant roles as pattern recognition receptors (PRRs) in the innate immunity. In this study, a novel C-type lectin with four dissimilar carbohydrate-recognition domains (CRDs) was identified from Argopecten irradians (designated as AiCTL-9). The full-length cDNA of AiCTL-9 was of 2291 bp with an open reading frame of 1827 bp encoding a polypeptide of 608 amino acids with a signal sequence and four CRDs. The motifs determining carbohydrate binding specificity in each CRD of AiCTL-9 were different, and they were YPT in CRD1, EPD in CRD2, EPN in CRD3 and QPN in CRD4, respectively. All the four CRDs shared the similar potential tertiary structure of a typical double-loop structure with Ca2+-binding site 2 in the long loop region and two conserved disulfide bridges at the bases of the loops. The mRNA transcripts of AiCTL-9 were mainly detected in hepatopancreas as well as gonad, and also marginally detectable in mantle, adductor, gill and hemocytes. Its relative expression level in hemocytes was significantly up-regulated after the challenges of fungi Pichia pastoris GS115 (P < 0.05). Gram-positive bacteria Micrococcus luteus (P < 0.05) and Gram-negative bacteria Vibrio anguillarum (P < 0.01). The recombinant AiCTL-9 (rAiCTL-9) could bind various PAMPs, including LPS, PGN, mannan and glucan, and also displayed agglutinating activity to fungi P. pastoris GS115, Gram-positive bacteria Bacillus subtilis and Gram-negative bacteria Escherichia coli TOP10P as well as V. anguillarum in a Ca2+ dependent manner. Moreover, rAiCTL-9 could initiate the cellular adhesion of hemocytes and enhance their encapsulation in vitro. All these results implied that AiCTL-9 was a novel PRR involved in immune response of scallop against a large number of pathogens by recognizing different PAMPs and enhancing scallop hemocytes encapsulation. (c) 2011 Elsevier Ltd. All rights reserved.
关键词	Argopecten Irradians C-type Lectin Innate Immunity Pattern Recognition Microbial Agglutinating Activity Cellular Adhesion
学科领域	Immunology ; Zoology
DOI	10.1016/j.dci.2011.10.002
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000301170800012
引用统计	被引频次：55[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12259
专题	实验海洋生物学重点实验室
通讯作者	Wang, LL (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Wang, Leilei,Wang, Lingling,Yang, Jialong,et al. A multi-CRD C-type lectin with broad recognition spectrum and cellular adhesion from Argopecten irradians[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2012,36(3):591-601.
APA	Wang, Leilei.,Wang, Lingling.,Yang, Jialong.,Zhang, Huan.,Huang, Mengmeng.,...&Wang, LL .(2012).A multi-CRD C-type lectin with broad recognition spectrum and cellular adhesion from Argopecten irradians.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,36(3),591-601.
MLA	Wang, Leilei,et al."A multi-CRD C-type lectin with broad recognition spectrum and cellular adhesion from Argopecten irradians".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 36.3(2012):591-601.

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